[Food & Water Safety][렙토스피라균 검출 qPCR 키트] Leptospira spp. (16S rDNA) qPCR Kit [MD06271]_NZYTech - 코아사이언스

 

Leptospira spp. (16S rDNA) qPCR Kit

Cat.# MD06271 / Size: 100 reactions

Manual / MSDS

Leptospira spp. (16S rDNA) qPCR Kit is designed for the in vitro detection of pathogenic and intermediately pathogenic Leptospira genomes.

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Leptospira is a Gram-negative bacterium that belongs to the Leptospiraceae family. Leptospirosis is a worldwide zoonosis affecting many wild and domestic animals. Common clinical signs include fever, vomiting, abdominal pain, diarrhea, severe weakness and depression, stiffness, severe muscle pain and inability to have offspring. The disease causes economic losses as it affects livestock, with decreased milk production and death. Humans can be infected by contact with the urine of infected animals.

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Leptospira spp. (16S rDNA) qPCR Kit is designed for the in vitro detection of pathogenic and intermediately pathogenic Leptospira genomes. The kit is built to have the broadest possible detection profile whilst remaining specific to this species. Thus, this kit has been designed for the specific (inclusivity) and exclusive (exclusivity) in vitro detection of Leptospira species. The primers and probe sequences have very high (>95%) homology with a broad range of Leptospira genomes based on a comprehensive bioinformatic analysis with all reference data within the NCBI database. Other closely related genus are not detected.

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If required, a complementary kit for the detection of an endogenous gene of the species from which samples are being extracted is available at NZYTech (see Vet, Food & Pharma and Human). The complementary usage of an Endogenous Detection reaction provides a solid confirmation that nucleic acids were properly extracted from the selected biological matrix. If you require further information or have a specific question about the detection profile of this kit, please send an e-mail to info@nzytech.com and our scientific team will answer your question. This kit is designed to be used by trained users in a suitable molecular biology laboratory environment.

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Components:

- Lyo NZYSupreme qPCR master mix (2x)

- qPCR master mix reconstitution buffer

- Lyo PPMix (10x)

- NTC

- Positive Control

- DNA Internal Extraction Control (IEC)

 

Shipping Conditions	Room Temperature
Storage Conditions	-85 °C to -15 °C
Format	Lyophilized
Product Category	Predesigned qPCR assays
Compatibility	All standard Real-time thermal cyclers
Target Species	Leptospira spp. (16S rDNA)
Sample Material	DNA
Protocol time	Approx. 60 min (from extracted NA to result)
Number of targets	Duplex
Internal Control	No
Features	Free automatic data analyzer (Fastfinder)
Detection Method	Probe Based

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