[소 배아 동결 보존 용액] CryoBov™ for Embryo [BPL-B1]_Funakoshi - 코아사이언스

 

CryoBov™ for Embryo

Cat.# BPL-B1 / Size: 8 mL

Datasheet

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*Letter of Assurance (LoA) is required for purchase

CPLL solution for bovine embryo freeze reagent

Contains carboxylated poly-L-lysine (CPLL)

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Storage: 2-10℃

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Feature:

• CryoBov™ for Embryo is a mixed solution of ethylene glycol, propanediol and CPLL, etc.
• This product can be used as a slow freezing (direct method) solution for bovine embryo by adding 20% serum according to the protocol.
• Free of bacteria and fungi contamination.
• The product is stable for 1 year at 2-10oC at stability after the date of manufacture.

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About carboxylated poly-L-lysine (CPLL)

1. Carboxylated poly-L-lysine (CPLL) is the new amphoteric electrolyte high molecular compound which introduced the carboxyl group (-COOH) by making a part of amino group of ε-poly-L-lysine (PLL) currently used as a food additive react.

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2. Molecular weight of CPLL is about 4,000-5,000. Since it is a high molecular compound, It does not penetrate a cell membrane, but it is thought that I have reduced the cell damage from the physical cell obstacle by freeze (icy crystal) cell outside by sticking to a cell membrane at the time of a freeze.

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3. CPLL has a function similar to anti-freeze protein (special protein which the fishes which inhabit a polar zone, and a plant hold in the living body), and It can control the re-crystallization at the time of growth (freeze concentration) of an ice crystal, or dissolution out of a cell.

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4. Cryopreservation solution using CPLL shows that It is effective in cryopreservation of various cells by traditional research.

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Example of an experiment

Conception rate evaluation results

After an ovum fertilized in vivo of Japanese Black Bovine cryopreserved by Conventional method and New method with CPLL were stored in liquid nitrogen for more than one week, transferred them to Japanese Black Bovines and Holsteins, and the conception rate was evaluated after 40 to 50 days.

The conception rate evaluated in 81 Black Japanese Bovines (Conventional method 31 bovines and New method with CPLL 50 bovines) was 29.03% by Conventional method and 50.00% by New method.

The conception rate evaluated in 61 Holsteins was 33.33% by Conventional method and 39.53% by New method.

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※All products listed in this paper are sold for research purposes only. They are not intended for use in pharmaceuticals, diagnostic pharmaceuticals, food products, or food testing. They should not be used on the human body regardless of their intended use, such as in the manufacture of pharmaceuticals, quality control, various diagnoses, and treatment.

 

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