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NZYMiniprep 96 well plate

Cat.# MB20201 / Size: 2 plates

Cat.# MB20202 / Size: 4 x 2 plates

Manual / MSDS

NZYMiniprep 96 well plate kit is designed for the manual or automated rapid, small-scale preparation of highly pure plasmid DNA from E. coli strains.

NZYMiniprep 96 well plate kit is designed for the manual or automated rapid, small-scale preparation of highly pure plasmid DNA from recombinant Escherichia coli strains. The method is based on the alkaline lysis of bacterial cells followed by adsorption of DNA onto silica in the presence of high salt. The plasmid DNA is selectively adsorbed in the silica gel-based NZYMiniprep 96 well plate and other impurities such as proteins, salts, nucleotides and oligos (<40-mer) are washed away. NZYMiniprep 96 well plate kit includes an additional washing buffer (AY) which is strongly recommended for the complete removal of high levels of endonucleases. NZYMiniprep 96 well plate kit allows simultaneous processing of up to 96 samples, typically in less than 45 min. Actual processing times may depend on the configuration of the liquid handling system used. The final concentration of the eluted DNA is 50–200 ng/μL (depending on the elution buffer volume and the bacterial culture). The kit is designed for use with vacuum manifolds or under centrifugation.

Components:

– Buffer A1

– Buffer A2

– Buffer A3

– Buffer AY

– Buffer A4 (concentrate)

– Buffer AE (does not contain EDTA)

– RNase A (lyophilised)

– NZY Plasmid filter plate (violet rings)

– NZY Plasmid binding plate (white rings)

– Elution plate (V– bottom)

– 96– deep– well block (1.2 mL)

 

Shipping Conditions
Room Temperature
Storage Conditions
15 °C to 25 °C
Starting Material
E. coli culture
Scale
Miniprep
Features
High-throughput (Vacuum manifold), Plasmid type: high-, low-copy number
Format
Plate
Product Category
DNA Purification, Plasmid DNA Purification
Isolation Technology
Silica membrane
Protocol time
Approx. 45 min
 

* 본 상품은 오직 연구용으로만 사용 가능합니다. 인체 및 제품화에 사용하실 수 없습니다.

코아사이언스 coresciences NZYTech, Lda. genes & enzymes korea 한국 molecular biology 분자생물학 enzymes & proteins 효소 단백질 assay kits 분석 측정 테스트 키트