[식물/곰팡이 유전체 DNA 추출 키트] NZY Plant/Fungi gDNA Isolation kit [MB17701, MB17702]_NZYTech - 코아사이언스

NZY Plant/Fungi gDNA Isolation kit

Cat.# MB17701 / Size: 50 columns

Cat.# MB17702 / Size: 4 x 50 columns

Manual / MSDS

NZY Plant/Fungi gDNA Isolation kits are designed for the simple and rapid small-scale preparation of highly pure genomic DNA from plant tissues and fungi samples by means of silica-based spin column.

​

NZY Plant/Fungi gDNA Isolation kits are designed for the simple and rapid small-scale preparation of highly pure genomic DNA from plant tissues and fungi samples. The method is spin column silica-based and requires no phenol or chloroform extraction. This kit uses two optimized lysis buffers systems based on the established CTAB and SDS methods. NZY Homogenization columns are included in this kit for conveniently clearing the crude lysates. The clear flow-through is mixed with Binding buffer PN for optimal binding of DNA to the silica membrane. Then, the DNA is selectively absorbed into the NZYSpin Plant Column and others impurities such as proteins and salts are removed during the washing steps.

The eluted genomic DNA has a A260/280 ratio between 1.7 and 1.9 what makes it ready to use in applications like sequencing, PCR, multiplex-PCR, genotyping and a wide range of other enzymatic manipulations.

​

Components:

– Buffer PNL1

– Buffer PNL2

– Buffer PNL3

– Buffer PN

– Buffer PNW1

– Buffer PNW2 (concentrate)

– Buffer PNE

– RNase A(lyophilized)

– NZYHomogenization columns (purple rings)

– NZYSpin Plant columns (green rings)

– Collection tubes (2 mL)

​

Applications:

Genotyping, PCR, qPCR, Sequencing

​

Citations:

Toward alternative sources of milk coagulants for cheese manufacturing: establishment of hairy roots culture and protease characterization from Cynara cardunculus L

Folgado A, Pires AS, Figueiredo AC, Pimentel C, Abranches R.

Plant Cell Rep., 2020

 

Shipping Conditions	Room Temperature
Storage Conditions	15 °C to 25 °C
Starting Material	Fungi, Plant tissue
Scale	Miniprep
Features	Manual
Format	Column
Product Category	DNA Purification, Genomic DNA Purification
Isolation Technology	Silica membrane
Protocol time	Approx. 30 min

* 본 상품은 오직 연구용으로만 사용 가능합니다. 인체 및 제품화에 사용하실 수 없습니다.

코아사이언스 coresciences NZYTech, Lda. genes & enzymes korea 한국 molecular biology 분자생물학 enzymes & proteins 효소 단백질 assay kits 분석 측정 테스트 키트