"Prote-in" Transfection Reagent - Peptide-base Protein Transfection Reagent for Cells [160-0526-0, 160-0518-0]_Funakoshi - 코아사이언스

Peptide-base Protein Transfection Reagent for Cells

"Prote-in" Transfection Reagent

Ready to use and suitable replacement of trypsin

Cat.# 160-0526-0 / Size: 6 tests

Cat.# 160-0518-0 / Size: 24 tests

본 상품은 펩타이드를 기반으로 한 세포 내로의 단백질 형질전환 시약입니다. 세포막 투과정이 매우 우수하며 단백질-운반체 복합체 형성이 잘 됩니다.

 

"Prote-in" is a peptide-base protein transfection reagent for cells. This reagent has the excellent ability of membrane penetration and formulation of protein-transporter complex.

Peptide transporter "Prote-in" consists of 3 region of peptide sequences - Hydrophobic region, Linker and Cell Penetrating Peptide (CPP) area. Hydrophobic region binds to Proteins. CPP region helps to corporate the protein-transporter complex into cytoplasm by binding to heparan sulfate proteoglycan on the cell surface. As transporter and protein do not bind covalently, protein function is maintained.

Features of "Prote-in" 

• Low cytotoxicity
• Rapid - Complex is structured in an hour, and transfection is completed in 3 hours.
• Versatility - Can be used for both Adherent and Non-adherent Cells.

Principle of "Prote-in" 

Protocol Outline  

Example of Use 

HeLa-S3 Cell Line (Human Uterus Cancer : Adherent Cell)

FITC labeled Antibody : Anti-Sheep IgG (Whole Molecule), FITC Conjugate, Donkey Polyclonal (SIGMA)

Examples of Cells Transfected Successfully 

Adherent Cells	Non - Adherent Cells
HeLa-S3 cell line	K562 cell line
WiDr cell line	Jurkat cell line
HepG2 cell line	HL60 cell line
MCF-7 cell line	P388 cell
Balb3T3 cell line	P3U1 cell
B16 cell line
SVT2 cell line
A10 cell line

Comparison Data 

References

1. Eisaku et al., High efficient delivery of p16 antitumor peptide into aggressive leukemia/lymphoma cells using a novel transporter system. Mol. Cancer Ther., 3(12),1623-1630 (2004). 

2. Eisaku et al., Potent synergy of dual antitumor peptides for growth suppression of human glioblastoma cell lines. Mol. Cancer Ther., 7(6), 1461-1471 (2008). 

* FOR RESEARCH USE onLY. NOT FOR DIAGNOSTIC USE.

코아사이언스 coresciences innovative cell technologies Funakoshi Inc. Japan 후나코시 한국 대리점 hygieiabio.co.jp hygieia bioscience 단백질 형질전환 형질감염 protein transfection 세포 내 단백질 도입 전달 막단백질 통과