BCAS1 antibody [445 004]_Synaptic Systems- 코아사이언스

BCAS1 antibody

Guinea pig polyclonal antiserum

Cat. # 445 004 / Size: 100 ul

 

BCAS1 is a protein specific for an actively myelinating oligodendrocyte subpopulation

 

Cat. No. 445 004	100 µl antiserum, lyophilized. For reconstitution add 100 µl H2O, then aliquot and store at -20°C until use.
Applications	WB: 1 : 1000 (AP staining) (see remarks) gallery   IP: not tested yet ICC: 1 : 500 gallery   IHC: 1 : 500 gallery   IHC-P/FFPE: 1 : 1000 gallery
Immunogen	Mixture of two synthetic peptides corresponding to AA 305-327 and AA 360–379 in rat BCAS1
Reactivity	Reacts with: mouse (Q80YN3), rat (Q3ZB98). Other species not tested yet.
Specificity	Specific for BCAS1 and its isoforms
Remarks	WB: shows almost no signal in westernblots on mouse brain lysate.

Background

Breast carcinoma-amplified sequence 1 (BCAS1) was originally identified as a potential oncogene amplified in human cancer cell lines (1). It is also highly expressed in brain (2) where it mainly defines an oligodendroglial subpopulation occuring at regions of active myelin formation (3).
In fetal brain BCAS1 positive oligodendrocytes are restricted to the white matter. In adult brains they persist in the grey matter until old age (3).

1) Collins C, Proceedings of the National Academy of Sciences of the United States of America (1998) 9515: 8703-8.
2) Ishimoto T, Glia (2017) 655: 727-739.
3) Fard MK, Science translational medicine (2017) 9419: .

Protocols

WB

• AP Detection
• ECL Detection
• Fluorecent detection

ICC

• PFA fixation

IHC

• Standard protocol
• Tissue preparation

IHC-P

• Chromogenic detection
• Fluorescent detection
• Tissue preparation

 

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