[Solutions for Evaluation of CAR Expression] CAR Detection Strategy and Product Design_ACROBiosystems 한국 수입공급 전문 - 코아사이언스

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표적 치료제, 진단, 백신 연구관련 시약 및 재조합단백질 전문 제조사 ACROBiosystems 공식 수입/공급 업체 코아사이언스 입니다.

 

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CAR Detection Strategy and Product Design

 

Direct method —Fluorescent-Labeled Proteins

Key Features 

• Target antigens are pre-labeled with a fluorescent dye.
• Processing time can be reduced by the use of direct-labeled proteins.
• Non-specific reaction of a secondary antibody is eliminated.

 

> PE-labeled （Click molecule view product details） -> Specially designed

Her2       CD4      Anti-FMC63 Ab      BCMA      CD19       MSLN       CD33      CLL-1      SLAMF7     CD30     CD7     EGF R     EGFRvIII      GPC3      GUCY2C      ROR1  

 

> FITC-labeled （Click molecule view product details） -> Specially designed

Anti-FMC63 Ab    BCMA     CD123    CD138    CD19    CD22     CD30    CD38     CD4    CD8A & CD8B    CD70    CLL-1    EGF R     EGFRvIII    FAP    FOLR1    GPC3     GUCY2C     Her2    Her3    IL13RA2    MSLN    PSMA    ROR1    SLAMF7     CD56     B7-H3     EpCAM     CD147     CD7    IL-13 R alpha 2    Nectin-4    CD5    

 

> Case Study

Evaluation of Anti-CD19 CAR Expression with FITC-labeled CD19

293 cells were transfected with anti-CD19-scFv and RFP tag. 2e5 of the cells were stained with B. FITC-Labeled Human CD19 (20-291) (Cat. No. CD9-HF2H2, 10 µg/ml) and C. FITC-labeled protein control. A. Non-transfected 293 cells and C. FITC-labeled protein control were used as negative control. RFP was used to evaluate CAR (anti-CD19-scFv) expression and FITC was used to evaluate the binding activity of FITC-labeled Human CD19 (20-291) (Cat. No. CD9-HF2H2).

 

> Application — Evaluation of CAR Expression

Evaluation of CAR Expression - ACROBiosystems

 

Biotin-streptavidin based detection using biotinylated proteins

• Target antigens are pre-labeled with biotin and detected by labeled streptavidin (the biotin-avidin complex).
• Streptavidin labeled with fluorochromes can bind biotinylated proteins with a high degree of affinity and specificity, amplifying the signal and improving the detection sensitivity and specificity.

 

> Biotinylated proteins -> Specially designed

Anti-FMC63 Ab   B7-H3     BCMA     CD138    CD147    CD19    CD22    CD30    CD33    CD38    CD4   CD70    CEA    EGF R    EGFRVIII    EpCAM    FAP    FOLR1     GPC3    Her2    Her3    HGFR    MSLN     MUC16   Nectin-4    PSMA     ROR1    SLAMF7    uPAR     VEGFR2     CD56     CD7     CD5     IL-13 R alpha 2

 

> Case Study

Evaluation of Anti-BCMA CAR Expression with Biotinylated BCMA

Human T cells were transfected with anti-BCMA CAR and cultured for 3 days. Three days post-transfection, 1e6 cells were first incubated with 50 µl biotinylated human BCMA protein (Cat. No. BC7-H82F0, 8 µg/ml), washed and then stained with PE Streptavidin and analyzed by flow cytometry. (Data are kindly provided by PREGENE Biopharma)

 

> Application — Evaluation of CAR Expression

 

Indirect detection using unconjugated proteins

• Target antigens are designed to carry a specific tag and detected using a secondary antibody (anti-epitope tag antibody) labeled with a fluorophore.
• Non-specific reaction of a secondary antibody may occur.

 

> Unconjugated proteins -> Specially designed

Anti-FMC63 Ab    B7-H3    BCMA    CAIX    CD123    CD133    CD138    CD147    CD19    CD22    CD30    CD33    CD38    CD4    CD7    CD70    CEA     EGF R    EGFRVIII    EpCAM    FAP    FOLR1    GPC3    Her2    Her3    HGFR     IL13RA2    MSLN     MUC1    Nectin-4     NKG2D    PSCA    PSMA    ROR1    SLAMF7    uPAR     VEGFR2

 

> Case Study

Evaluation of Anti-CD19 CAR Expression with Fc-fusion CD19

293 cells were transfected with FMC63-scFv and RFP tag. 2e5 of the cells were first stained with B. Human CD19 (20-291) Protein, Fc Tag, low endotoxin (Super affinity) (Cat. No. CD9-H5251 , 3 µg/ml) and C. Human Fc Tag Protein Control, followed by FITC-conjugated Anti-human IgG Fc Antibody. A. Non-transfected 293 cells and C. Human Fc Tag Protein Control were used as negative control. RFP was used to evaluate CAR (anti-CD19-scFv) expression and FITC was used to evaluate the binding activity of Human CD19 (20-291) Protein, Fc Tag, low endotoxin (Super affinity) (Cat. No. CD9-H5251).

 

> Application — Evaluation of CAR Expression

 

More CAR-T related products

> Click here to learn more about mAb for anti-CD19（FMC63）CAR detection-flow cytometry validated

> Click here to learn more about mAb for anti-CD20 CAR detection-flow cytometry validated

> Click here to learn more about high affinity CD19 protein

 

추천 상품 

[가스 투과성 세포 배양 백] NIPRO Cell Culture Bags [87-352, 87-302] & Connecting Tube [87-360]

[마이크로플레이트 웰에서 효과적인 세포 회수/소형 셀 스크래퍼] Mini Cell Scraper: Best size for 24, 48 and 96 well culture plates [MCS-200]

 

미니 셀 스트래이너/미니 셀 스트레이너/Single cell 분리] Mini Cell Strainers for 5 ml sampling tubes and 15 ml conical tubes [HT-AMS-12502, HT-AMS-14002, HT-AMS-17002] & Mini Cell Strainer II for 1.5 mL/2.0 mL tube and 5 ml sampling tubes [HT-AMS-04002, HT-AMS-04008]

코아사이언스 coresciences Acrobiosystems Acro biosystems 면역치료제 immuno cell therapy targeted therapy cytokines 사이토카인 recombinant proteins 재조합 단백질 인터페론 CART T cell B-cell natural killer cell 자연살상세포 항체 antibody 면역학 immunology