[2세대 LipiDye(리피다이)/High sensitive imaging dye for lipid droplets] LipiDye II [FDV-0027]_Funakoshi -코아사이언스

LipiDye II

Live imaging fluorescent dye for lipid droplets (LDs)

Cat.# FDV-0027 / Size: 0.1mg

LipiDye II 1세대 LipiDye보다 뛰어난 phoostability로 장시간 live cell imaging에 더욱 최적화 되었습니다!

 

LipiDye II is a high sensitive, low cytotoxic and super-photostable fluorescent dye for Lipid Droplets (LDs). 

LipiDye II enables to detect very small LDs (less than 1µm) and to perform long-term time-lapse imaging, including Z-stack imaging. This novel dye succeeded in observation of dynamic biosynthesis, degradation and movement of LDs in live cells.

 

Background 

What is lipid droplets (LDs)?

Lipid droplets (LDs) are organelles that have unique phospholipid monolayer and store neutral lipids such as triglycerides and sterol esters (Below figure left). LDs are historically found in adipose tissue and considered as sites for energy storage or lipid turnover. Recent studies discovered that LDs are not only in adipocytes, but also found ubiquitously in cells from yeast to mammalian cells. The numbers, size and composition of LDs largely differ depending on cell types or even within the same cell. For example a dipocytes usually have large LD structures (>10μm) which can be observed by optical microscopy. On the other hand, non adipocytes have a much smaller LD structure compared with adipocytes (Below figure right).

LDs are produced from the endoplasmic reticulum (ER), exported to the cytoplasm and expand via fusion of LDs or incorporation of additionally synthesized neutral lipids. LDs contact with various organelles including ER, mitochondrias, lysosomes, nucleus and shows dynamic movement inside the cells.

 

Problem of conventional LD dyes: stability, sensitively, selectivity etc...

To observe the dynamic movement of LDs in live cells and investigate the physiological functions of LDs, a specific LD dye compatible with long term live cell imaging is required. Conventional fluorescent dyes for LDs such as Nile Red contribute to elucidate biological functions of LDs but its sensitivity and selectivity are limited to detect relatively large LDs in adipocytes or cells treated with excess lipids. It is challenging for conventional dyes to detect small LDs often founds in non-adipocytes under live cell conditions. Funakoshi provides a green fluorescent dye LipiDye (catalog no. #FDV-0010) which shows high sensitivity and selectivity for LDs and can detect approximately 1 μm size s of LDs. Although LipiDye is a powerful tool to monitor small LDs in non-adipocytes, LipiDye requires 405 nm excitation and has insufficient photostability, not suitable for long-term live cell imaging to observe dynamic LDs synthesis, movement or degradation.

 

LipiDye II is an innovative dye for advanced LD research

Here,LipiDye II , an upgrade version of LipiDye , can be excited by less toxic 450-480 nm light and exhibits super photostability. LipiDyeII is very suitable for long-term live cell imaging including Z-stack time-lapse imaging with multiple time excitations for short term intervals. For example, LipiDye II was applied in long-term imaging for drug induced LD degradation processes for 12 hours with 3,000 image captures and visualized lipolysis of LDs and de novo synthesis of very small (<1 μm) LDs. Furthermore, LipiDye II is compatible with STED microscopy and enables detecting less than 500 nm LDs in HeLa cells.

 

Features 

- High S/N ratio: Low background in cytoplasm via following two features

1) Specific accumulation into LDs

2) Strong green fluorecent emission in non-polar oil phase like LDs

- High sensitivity: Can observe even very small (<1 μm) LDs

- Super photo-stability: Long-term live time lapse imaging is possible

- Low cytotoxicity (at the recommended concentration (0.1～1μM))

- Compatible with both live and fixed cell

- Applicable to STED microscopy: can detect less than 500 nm LDs in HeLa cells.

 

Fluorescent characteristics 

Ex. 400-500 nm (maximum ~420nm)
Compatible with blue excitation lasers (ex. 405, 445, 458, 473 and 488 nm lasers, etc.), Xenon lamp or LED with commercial FITC or GFP filters.
*Note
488nm laser can excite LipiDye II but shows weak fluorescence compared with 473 nm excitation. If using 488 nm laser, please empirically optimize imaging conditions such as dye concentration etc. for your experiments.

Em. 450-650 nm (depend ent on solvents)
Maximum ~510 nm in soybean oil similar to LDs. Around 490-550 nm range is recommended.

 

Superiority of LipiDye 

코아사이언스 coresciences Funakoshi Inc. Japan 후나코시 한국 대리점 세포 지질 세포 내 지방소립  지방 방울 Lipid Droplet 이미징 형광 염색 시약 염색약 염색 형광물질 지방방울 지방소립 염색약 시약 합성 분해 이동 생동학 분석 살아있는 세포 내에서