Tissue Clearing Reagent Kit CUBIC [CSCR001, CSCR002, CSCR003,CSCR004, CSCR006, CSCR015, CSCR016, CSCR011, CSCR012]_Funakoshi - 코아사이언스

Enabling 3D Observation of Tissues through Clearing

Tissue Clearing Reagent Kit CUBIC

CUBIC is a water-soluble tissue clearing reagent kit designed for PFA-fixed organs and tissues. It enables 3D imaging of entire organs at cellular resolution using Light-Sheet Fluorescence Microscopy (LSFM) or Confocal Laser Scanning Microscopy (CLSM). The protocol requires only standard laboratory equipment—no specialized equipments like electrophoresis systems are required.

CUBIC Technology was developed by RIKEN and the University of Tokyo over about 10 years to allow observation of all cells in organs and the body. Today, it is used by researchers around the world and gained high trust as a versatile tissue clearing and 3D tissue staining method.

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※ Please click here for information on the 3D tissue staining kit CUBIC-HV

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Features

• Highly versatile water-soluble CUBIC tissue clearing kits that have a powerful transparency level.
• Compatible with all organs including the bone. Fluorescent protein signals can be observed after clearing the sample.
• High simplicity and reproducibility protocol solves issues pertaining to safety, waste processing, microscope compatibility, fluorescent protein signal retainability, etc.

​Product Lineup

CUBIC Basic Clearing Reagents

Animal organs can be cleared simply by immersing them in two types of reagents.

Click on a product code to view the price list for each item.

Product Name	Product Description	Cat.#
Tissue-Clearing Reagent CUBIC-L （for Animals）	For delipidation and decolorization.	CSCR001
Tissue-Clearing Reagent CUBIC-R+(N) （for Animals）	For Clearing (refractive index adjustment). It contains Nicotinamide and offers excellent versatility and handling.	CSCR002
Tissue-Clearing Reagent CUBIC-R+(M) （for Animals）	It contains N-methylnicotinamide and offers superior preservation of fluorescent protein signals.	CSCR004
CUBIC tissue clearing kit （CUBIC-L 250 ml/CUBIC-R+(N) 250 ml）	A trial set containing 250 ml each of CUBIC-L and CUBIC-R+(N).	CSCR006

※ 500 ml is sufficient for approximately 20 mouse brain samples.

※ CUBIC-R reagents are designed to expand the sample by approximately 1.5x along each axis to improve transparency and resolution during microscopic imaging.

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CUBIC Bone Tissue Clearing Reagent

This reagent is optimized for bone tissue clearing. It is designed to use in combination with CUBIC-L/R.

Click on a product code to view the price list for each item.

Product Name	Product Description	Cat.#
Tissue-Clearing Reagent CUBIC-B （for Animals）	A powerful bone clearing reagent optimized for bone tissue decalcification.	CSCR003

※ 100 ml is sufficient for approximately 5 to 10 mouse hindlimb samples.

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Clearing reagents for organoids, spheroids, and animal embryos

This reagent is optimized for organoids, spheroids, and animal embryos, based on CUBIC Basic Clearing Reagents (CUBIC-L/R).

Click on a product code to view the price list for each item.

Product Name	Product Description	Cat.#
CUBIC tissue clearing kit for Organoid, Spheroid and Embryo （CUBIC-Lf 50 ml/CUBIC-Rf 75 ml）	A set comprising a delipidation/decolorization reagent and a refractive index adjustment reagent. For approximately 48 organoid samples.	CSCR015
CUBIC tissue clearing Trial kit for Organoid, Spheroid and Embryo （CUBIC-Lf 25 ml/CUBIC-Rf 40 ml）	A set comprising a delipidation/decolorization reagent and a refractive index adjustment reagent. For approximately 24 organoid samples.	CSCR016

Related Product：Mounting Solution

To observe cleared samples using Light-Sheet Fluorescence Microscopy (LSFM) or Confocal Laser Scanning Microscopy (CLSM), the samples must be immersed in a mounting solution. This product is optimized for samples cleared with CUBIC-R+.

Click on a product code to view the price list for each item.

Product Name	Volume	Cat.#
Mounting Solution(RI 1.522)（for CUBIC-R+）	100 ml	CSCR011
	500 ml	CSCR012

How To Use

Application Examples

Adult mouse brain

For other application examples, please refer to the technical guidebook downloaded from Product Information.

※ Amount of reagent and treatment time varies depending on the organ.

※ Please use a slightly larger tube wider than the organ’s diameter. Most of the organ should be immersed in the reagent when the tube is laid on its side.

※ Shake the organ immersed in the reagent at a speed that shakes the whole organ.

※ We do not recommend rotation as it will create bubbles.

 

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