[Dyes & Labeling/Fluorescent Dyes/Rhodamine Derivatives/Amine-Reactive] 6-Carboxytetramethylrhodamine NHS ester, single isomer [AF-0108][CAS no. 136977-42-9]_emp Biotech - 코아사이언스

6-Carboxytetramethylrhodamine NHS ester, single isomer (6-TAMRA-SE)

Cat.# AF-0108-D005.0-001 / Size: 5mg

Cat.# AF-0108-D020.0-001 / Size: 20mg

Cat.# AF-0108-D100.0-001 / Size: 100mg

Amine-reactive rhodamine dye marker for fluorescence labeling, especially for labeling of biomolecules (Abs: 554 nm, Em: 577 nm).

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Properties

CAS-Number:	136977-42-9
Molecular Weight:	527,5
Empirical Formula:	C29H25N3O7
Absorption [nm]:	548 ± 3
Emission [nm]:	570 ± 3
ε [M-1 cm-1]:	>78 000
Buffer System for Fluorescence Readings:	PBS, pH 7.4
Solubility:	Acetonitril, DMF, DMSO
Appearance (colour):	violett
Appearance (form):	solid (powder)
Storage:	- 20 °C. Keep dry! Keep away from light, especially in solution!
Trade name:	6-Carboxytetramethylrhodamine, succinimidylester, single isomer; 6-TAMRA-SE; 6-TAMRA succinimidyl ester, singel isomer
Reactive Moiety:	NHS ester, reacts with 1° amines to form a stable amide linkage

Description

This single isomer amine-reactive red fluorescent derivatization reagent may be used for covalent labeling of proteins and alkylamino modified nucleic acids.

The fluorescence quantum yield of TAMRA conjugates is about one-fourth that of fluorescein conjugates. Nevertheless, TAMRA conjugates often appear brighter than the corresponding fluorescein conjugates due to the fact that TAMRA is readily excited by the 546 nm spectral line from mercury-arc lamps used in most fluorescence microscopes and is intrinsically more photostable than fluorescein. TAMRA is also efficiently excited by the 543 nm spectral line of the green He-Ne laser.

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6-TAMRA-SE

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