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Monocarboxymethylene Blue (MCMB)

Cat.# F-0202-D005.0-001 / Size: 5mg

Non-activated methylene blue redox marker for electro-active labeling, designed especially for labeling of biomolecules.

Properties

Molecular Weight:
441,9
Empirical Formula:
C18H21N3O6SCl
Absorption [nm]:
665 ± 3
Emission [nm]:
670 ± 3
ε [M-1 cm-1]:
>81 000
Buffer System for Fluorescence Readings:
PBS, pH 7.4
Solubility:
Acetonitrile
Appearance (colour):
dark blue
Appearance (form):
solid (powder)
Storage:
- 20 °C. Keep dry! Keep away from light, especially in solution!
Trade name:
Monocarboxymethylene blue; Methylene blue
Reactive Moiety:
free carboxylic acid

Description

This non-activated methylene blue derivative is used as an electroactive labeling reagent for covalent attachment to proteins and alkylamino modified nucleic acids. Monocarboxymethylene blue contains a single carboxylic acid functional group. In addition to its relatively high absorption,MCMB has excellent and validated redox properties and is a good generator for singlet oxygen, making it to a particularly suitable label for electrochemical and photosensitzer applications.

CAS: none known

Formal Electrode Potential vs. Ag/AgCl (1 M KCl): Ef = -0.198 V

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