[Dyes & Labeling] Hoechst 33342, 20 mM in water [F-0409-M005.0-001][CAS no. 23491-52-3]_emp Biotech - 코아사이언스

 

Hoechst 33342, 20 mM in water

Cat.# F-0409-M005.0-001 / Size: 5mg

 

Blue nuclear counterstain for fixed- and live-cell fluorescence microscopy and cellular imaging (Ex: 346 nm and 361 nm, Em: 497 nm).

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Properties

CAS-Number:	23491-52-3
Molecular Weight:	615,98
Empirical Formula:	C27H37N6O4Cl3
Absorption [nm]:	343 ± 3
Emission [nm]:	497 ± 3
ε [M-1 cm-1]:	>45 000
Buffer System for Fluorescence Readings:	H2O
Solubility:	H2O
Appearance (colour):	yellow
Appearance (form):	solution
Storage:	+2-8 °C. Keep away from light!

Description

Hoechst 33342 from emp Biotech is a high-quality reagent for fixed- and live-cell fluorescent staining of DNA and cell nuclei, formulated specifically for cellular imaging techniques. It is primarily used for specifically staining nuclei of living or fixed cells and tissues.

Hoechst 33342 (2'-[4-ethoxyphenyl]-5-[4-methyl-1-piperazinyl]-2,5'-bi-1H-benzimidazole trihydrochloride trihydrate) is excited by UV light to emit a blue fluorescence between 460 to 490nm. It is cell-permeable and binds preferentially to adenine-thymine (A-T) regions and into the minor groove of DNA. In addition, Hoechst 33342 exhibits distinct fluorescence emission spectra dependent on dye:base pair ratios and is also commonly used in combination with:

5-bromo-2'-deoxyuridine (BrdU) # to characterize compact chromatin of apoptic nuclei, to classify replicating cells, and to sort cells subject to their DNA content

Propidium iodide # to distinguish various stages of apoptosis and cell-cycle distribution with simultaneous flow cytometric and fluorescence imaging analysis

Hoechst 33342 Aqueous Solution is compatible with antibodies and other probes which have been labeled with fluorescein and rhodamine, and can be used in tandem as a counterstain with such probes.

Excitation: 346 nm (361 nm with dsDNA)

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