Pertussis Toxin (백일해균 독소; 백일해 독소) [HB4729][CAS no. 70323-44-3]_Hello Bio - 코아사이언스

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Pertussis Toxin

Cat.# HB4729 / Size: 50ug

 

 

Product overview

Name	Pertussis Toxin

Alternative names

PTX | PT | Islet-activating protein | Holotoxin

Purity

>98%

Description

Catalyzes ADP-ribosylation of the G proteins Gαi, Gαo and Gαt

Biological Data

Biological description

Toxin produced by Bordetella pertussis . Catalyzes ADP-ribosylation of the α subunits of the heterotrimeric Gi/o proteins; Gαi, Gαo and Gαt . Blocks receptor coupling and activation by preventing G protein heterotrimers from interacting with receptors. This product is not activated. Cells will activate pertussis toxin in an intact system however activation is required in a cell free system. Incubation with high concentrations of dithiothreitol (DTT) can achieve activation, see Kaslow, et al. (1987) for suggested conditions.

Solubility & Handling

Storage instructions

+4°C for lyophilised or resuspended (do not freeze)

Handling

Use solutions within 1 month, long term storage is not recommended. Before use the suspension should be gently mixed (not vortexed) to make the suspension uniform. Do not sterile filter. Pertussis toxin can be inactivated by boiling for 30mins

Important

This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.

Chemical Data

Purity

>98%

CAS Number

70323-44-3

Source

B. pertussis

Appearance

Lyophilised

Formulation

Contains 0.05M sodium phosphate and 0.5M sodium chloride at pH 7.2.

References for Pertussis Toxin

References are publications that support the biological activity of the product

• G(i/o) protein-dependent and -independent actions of Pertussis Toxin (PTX).
• Mangmool and Kurose (2011) Toxins (Basel) 3(7) : 884-99 Read more (PubMedID: 22069745)
• Structure-activity analysis of the activation of pertussis toxin.
• Kaslow et al (1987) Biochemistry 26(1) : 123-7 Read more (PubMedID: 3030399)
• Subunit structure of islet-activating protein, pertussis toxin, in conformity with the A-B model.
• Tamura et al (1982) Biochemistry 21(22) : 5516-22. Read more (PubMedID: 6293544)

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Publications

These publications cite the use of Pertussis Toxin purchased from Hello Bio:

Differential activation of G protein-mediated signaling by synthetic cannabinoid receptor agonists

Sachdev S et al (2020) Pharmacol Res Perspect 8(2) : e00566

PubMedID: 32101383

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Cannabichromene is a cannabinoid CB2 receptor agonist

Udoh et al (2018) bioRxiv doi: : https://doi.org/10.1101/435057

 

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코아사이언스 coresciences Hellobio Hello bio UK distributor 한국 대리점 G protein coupled receptors agonist antagonist cell process cell signaling ionotropic receptors cell imaging Enzyme inhibitors Enzyme activators Stem cell modulators Receptor ligands Signaling tools Epigenetic tools Ion channel modulatorsLifesciences GmbH 독일 한국 대한민국 cell selection & expansion exosome isolation protein production & assay cell isolation staining antigen specific T cell isolation & staining T cell stimulation & expansion