[Aquaculture/수산양식 잉어봄바이러스병 분자진단] Spring Viremia of Carp virus RT-qPCR Kit [MD05681]_NZYTech - 코아사이언스

 

Spring Viremia of Carp virus RT-qPCR Kit

Cat.# MD05681 / Size: 100 reactions

 

Designed for the in vitro detection of SVCV. qPCR assay criteria are met, but PC is not meant for quantification.

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Details

Spring Viremia of Carp virus (SVCV) is a Rhabdovirus that causes an acute systemic, contagious disease primarily affecting carp. Spring Viremia of Carp is passed mainly horizontally, being shed in fecal casts, urine and gill mucus. It is also transmitted by louse, leech, crustacean and annelid vectors. The virus causes hemorrhage and inflammation of the swim bladder leading to abdominal distension, lethargy, imbalance, swimming on their side and sinking.

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Spring Viremia of Carp virus RT-qPCR Kit is designed for the in vitro detection of SVCV genomes. The kit is built to have the broadest possible detection profile whilst remaining specific to SVCV. Thus, this kit has been designed for the specific (inclusivity) and exclusive (exclusivity) in vitro detection of this species. The primers and probe sequences have very high (>95%) homology with a broad range of SVCV genomes based on a comprehensive bioinformatic analysis with all reference data within the NCBI database at the time of design. Due to the inherent instability of RNA viral genomes, it is not possible to guarantee the detection of all clinical isolates.

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This kit was meticulously designed and validated to meet the rigorous criteria of a quantitative assay. However, it is important to note that the provided Positive Control is not intended for quantification purposes. We recommend checking NZYtech website for the availability of a suitable Quantitative Standard for an accurate quantification. In alternative, commercially genomic DNA standards can also be used.

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If required, a complementary kit for the detection of an endogenous gene of the species from which samples are being extracted is available for purchase (see Vet, Food & Pharma). The complementary usage of an Endogenous Detection reaction provides a solid confirmation that nucleic acids were properly extracted from the selected biological matrix. If you require further information or have a specific question about the detection profile of this kit, please send an e-mail to info@NZYtech.com and our scientific team will answer your question. This kit is designed to be used by trained users in a suitable molecular biology laboratory environment.

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Components

- Lyo NZYSupreme One-step RT-qPCR Master Mix (2x)

- RT-qPCR master mix reconstitution buffer

- Lyo PPMix (10x)

- NTC

- Positive Control

- RNA Internal Extraction Control (IEC)

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Shipping Conditions	Room Temperature
Storage Conditions	-85 °C to -15 °C
Format	Lyophilized
Product Category	Predesigned qPCR assays
Compatibility	All standard Real-time thermal cyclers
Target Species	Spring Viremia of Carp virus
Sample Material	RNA
Protocol time	Approx. 60 min (from extracted NA to result)
Number of targets	Duplex
Internal Control	No
Detection Method	Probe Based

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