[Aquaculture/수산양식 분자진단] Photobacterium damselae ssp. piscicida qPCR Kit [MD05301]_NZYTech - 코아사이언스

 

Photobacterium damselae ssp. piscicida qPCR Kit

Cat.# MD05301 / Size: 100 reactions

 

Designed for the in vitro detection of P. damselae subs. piscicida. qPCR assay criteria are met, but PC is not meant for quantification.

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Details

Photobacterium damselae subspecies piscicida (previously known as Pasteurella piscicida) is a gram-negative rod-shaped bacterium that causes disease in fish, resulting in serious losses among cultured fish species in Europe.

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Photobacterium damselae ssp. piscicida qPCR Kit is designed for the in vitro detection of P. damselae ssp. piscicida genomes. The kit is built to have the broadest possible detection profile whilst remaining specific to P. damselae ssp. piscicida. Thus, this kit has been designed for the specific (inclusivity) and exclusive (exclusivity) in vitro detection of this subspecies. The primers and probe sequences have very high (>95%) homology with a broad range of P. damselae ssp. piscicida genomes based on a comprehensive bioinformatic analysis with all reference data within the NCBI database. Other closely related subspecies are not detected. Real-time PCR detection methods have been used in several studies to identify P. damselae ssp. piscicida genomes (Carraro R. et al 2018).

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This kit was meticulously designed and validated to meet the rigorous criteria of a quantitative assay. However, it is important to note that the provided Positive Control is not intended for quantification purposes. We recommend checking NZYtech website for the availability of a suitable Quantitative Standard for an accurate quantification. In alternative, commercially genomic DNA standards can also be used.

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If required, a complementary kit for the detection of an endogenous gene of the species from which samples are being extracted is available at NZYtech (see Vet, Food & Pharma). The complementary usage of an Endogenous Detection reaction provides a solid confirmation that nucleic acids were properly extracted from the selected biological matrix. If you require further information or have a specific question about the detection profile of this kit, please send an e-mail to info@NZYtech.com and our scientific team will answer your question. This kit is designed to be used by trained users in a suitable molecular biology laboratory environment.

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Components

- Lyo NZYSupreme qPCR master mix (2x)

- qPCR master mix reconstitution buffer

- Lyo PPMix (10x)

- NTC

- Positive Control

- DNA Internal Extraction Control (IEC)

 

Shipping Conditions	Room Temperature
Storage Conditions	-85 °C to -15 °C
Format	Lyophilized
Product Category	Predesigned qPCR assays
Compatibility	All standard Real-time thermal cyclers
Target Species	Photobacterium damselae subsp. piscicida
Sample Material	DNA
Protocol time	Approx. 60 min (from extracted NA to result)
Number of targets	Duplex
Internal Control	No
Detection Method	Probe Based

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