Rat Universal Reference Total RNA [RR-UR-100, RR-UR-200, RR-UR-400]_Zyagen - 코아사이언스

Rat Universal Reference Total RNA

Cat.# RR-UR-100 / Size: 0.1mg

Cat.# RR-UR-200 / Size: 2 x 0.1mg

Cat.# RR-UR-400 / Size: 4 x 0.1mg

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Product Description

Zyagen Rat Universal Reference RNA is a high-quality standard for gene expression analysis by microarray, prepared at industrial scales to minimize lot-to-lot variation. Reference RNA is composed of highly pure intact total RNA isolated of all tissues of several adult male and female Sprague Dawley rats.

RNA is isolated modified guanidine thiocyanate techniques which allows the rapid isolation of total RNA including microRNAs. After removing hair from skin and food from digestive tract, all tissues (organs) of animals were cut in small pieces, snap frozen, grinding to powder, and homogenized in RNA denatured solution. The extracted RNA is treated with RNase-free DNase-1 to remove residual DNA, precisely quantified by NanoDrop, and stored at -80oC.

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Features:

• High quality intact RNA without genomic DNA contamination

• Consistent control for standard data set comparisons

• Extracted from all male and female tissues for broad gene coverage

• Made at industrial scales to minimize lot-to-lot variation

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Quality Control:

The integrity of RNA sample as indicated by intact ribosomal RNA is verified by denatured agarose gel electrophoresis. The purity of RNA is assessed by spectrophotometer (A260/A280: 1.9-2.1). Residual DNA contamination is tested by PCR.

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Applications:

RNA is ideal for gene expression analysis by microarray, Northern blotting, ribonuclease protection assay, SI nuclease assay, RT-PCR/Q-PCR/RACE analysis, cDNA synthesis, RNA differential display, microRNA studies, and purification of mRNA for library construction.

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Packing/shipping:

Total RNA sample is routinely provided in RNase-free water at a concentration of 1mg/ml and shipped on dry ice for overnight delivery.

 

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