[Illumina & MGI platforms] RNA Seq Library Prep Kit [30055, 30056, 30057, 34056]_BioDynami - 코아사이언스

 

RNA Seq Library Prep Kit

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illumina platform (Protocol download / MSDS download)

Cat.#	30055S	30055L	30056S	30056L	30057S	30057L
Index type	non-index	non-index	index	index	unique dual index	unique dual index
Reactions	24	48	24	48	96	2 x 96

MGI platform (Protocol download / MSDS download)

Cat.#	34056	34056L
Index type	index	index
Reactions	24	96

The RNA Seq Library Prep Kit was developed for construction of high quality NGS libraries for next generation sequencing (illumina and MGI Platforms). RNA Sequencing is a very powerful tool to analyze transcriptome such as gene expression and transcription regulation, splicing characterization, mutation and variation detection etc. The kit needs purified RNA (example: rRNA depleted RNA or polyA mRNA) as input for library construction. Library multiplexing is possible with different types of indexes.

RNA Seq Library Prep Kit Workflow

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Three index types are available for the illumina platform kits:

Non-index (illumina Cat.# 30055): Libraries do not have index.

Index (illumina Cat.# 30056): A unique barcode sequence with 6 bases has been included in each of the index primers. RNA Sequencing library multiplexing is possible with up to 48 samples.

Unique dual index (illumina Cat.# 30057): RNA Sequencing library multiplexing up to 96 samples is possible with the unique dual indexes. We have developed a 4-Base Difference Index System. The system can generate indexes with at least 4 bases different from others in the 8-base indexing region. the unique dual indexing primers identify sequencing errors such as index hopping, mis-assignment, and de-multiplexing errors.

Indexes are available for the MGI platform kits (Cat.# 34112).

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Features

• Quick protocol
• Libraries will be ready in 2 hours
• Hands-on time is only ~10 minutes
• Guaranteed quality
• High yield
• Uniform coverage of transcripts
• Simple workflow
• Input purified RNA amount: From 3 ng to 100 ng

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Comparison of the protocol time: BioDynami RNA Seq Library Prep Kit vs other vendors’ kits. Hands-on time and walk-away time were indicated.

Library size and distribution of BioDynami kit, 20 ng and 3 ng of polyA mRNA as input.

Comparison of library yield and duplication rate under the same condition: 20 ng and 3 ng of polyA mRNA were used as input. PCR cycle numbers were indicated.

Comparison of coverage of transcripts: 20 ng of polyA mRNA were used as input. BioDynami kit has more uniform coverage.

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Related Products

• NGS DNA Library Prep Kit
• NGS DNA Library Prep Customization Kit
• PCR-free NGS DNA Library Prep Kit
• NGS Low Input DNA Library Prep Kit
• ChIP-Seq Library Prep Kit
• NGS Cell-free DNA Library Prep Kit
• NGS FFPE DNA Library Prep Kit
• Bisulfite Sequencing Library Prep Kit
• Methylation Specific Bisulfite-Seq Library Prep Kit
• NGS Single Stranded DNA Library Prep Kit
• NGS Ancient DNA Library Prep Kit
• NGS DNA Fragmentation & Library Prep Kit
• NGS DNA Library Prep Kit (Ion Torrent Platform)
• NGS DNA Fragmentation & Library Prep Kit (Ion Torrent Platform)

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코아사이언스 coresciences BioDynami Korea distributor 한국 대리점 NGS Library Preparation Targeted Sequencing DNA purification magnetic beads Nucleic acid extrasction DNA RNA Quantification DNA Fragmentation DNA ladder 핵산 분리 정제 추출 시퀀싱 차세대 염기서열 분석 키트 시약 마그네틱 비드