[Illumina & MGI platforms] NGS Cell Free DNA Library Kit [30029, 30031, 30033, 34031]_BioDynami - 코아사이언스

NGS Cell Free DNA Library Kit

illumina platform (Protocol download / MSDS download)

Cat.#	30029S	30029L	30031S	30031L	30033S	30033L
Index type	non-index	non-index	index	index	unique dual index	unique dual index
Reactions	24	48	24	48	96	2 x 96

MGI platform (Protocol download / MSDS download)

Cat.#	34031S	34031L
Index type	index	index
Reactions	24	96

 

The NGS Cell Free DNA Library Prep Kit (illumina and MGI Platforms) was developed for the construction of high quality cell-free DNA (cfDNA) libraries using 1 ng to 50 ng of cell-free DNA as input. The kit has a simple work flow and a fast procedure. Multiplexing of the cell free DNA library is possible based on the index type.

NGS Cell Free DNA Library Prep Kit Workflow

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The main source of cell-free DNA (cfDNA) is derived from apoptotic hematopoietic cells in blood and found in the plasma. The length of the cfDNA is about 150-200 bp in length. Circulating tumor DNA (ctDNA) derived from malignant tumors is a part of cfDNA. Both cfDNA and ctDNA can be used as a noninvasive biomarker since it offers a better approach in comparison to invasive tissue biopsies.

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NGS has been used for cfDNA and ctDNA sequencing in the field of liquid biopsy as it provides a whole genome level of molecular profiling. One of the hurdles for cfDNA sequencing is the difficulty of library preparation from the limited amount of cfDNA obtained from plasma. Our cell-free NGS kit makes it easy to get enough libraries from limited input in just 1.5 hours.

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Three index types are available for the NGS Cell Free DNA Library Prep Kit of the illumina platform:

Non-index (Cat.# 30029): Libraries do not have index.

Index(Cat.# 30031): Each of our index primers contains a unique 6-base index sequence that can be used for sample identification. Total 48 library multiplexing is possible.

Unique dual index (Cat.# 30033): Cell-free DNA library multiplexing up to 96 samples is possible with the unique dual indexes. We have developed a Four-Base Difference Index System. The system have at least 4 bases different from each other in the 8 bases index length. The primers effectively minimize sequencing errors such as mis-assignment, index hopping, index contamination etc.

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Indexes are available for the MGI platform kits (Cat.# 34031).

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Kit advantages:

• Fast and simple protocol
• Hands-on time is around 10 minutes
• The total protocol time is only 1.5 hours
• Easy procedure based on:
• Ready-to-use master mix for easy setup of reactions
• Less reaction components to simplify bench work
• Less magnetic beads used for cleanup procedures: This can reduce more than 50% of the beads consumption
• Guaranteed high library conversion efficiency
• Low input cell-free DNA: From 1 ng to 50 ng

 

Comparison of library conversion efficiency with different samples under the same condition.

Comparison of library yield with different samples under the same condition.

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Related Products

• NGS DNA Library Prep Kit
• NGS DNA Library Prep Customization Kit
• PCR-free NGS DNA Library Prep Kit
• NGS Low Input DNA Library Prep Kit
• ChIP-Seq Library Prep Kit
• NGS FFPE DNA Library Prep Kit
• Bisulfite Sequencing Library Prep Kit
• Methylation Specific Bisulfite-Seq Library Prep Kit
• NGS Single Stranded DNA Library Prep Kit
• NGS Ancient DNA Library Prep Kit
• NGS DNA Fragmentation & Library Prep Kit
• RNA Seq Library Prep Kit
• NGS DNA Library Prep Kit (Ion Torrent Platform)
• NGS DNA Fragmentation & Library Prep Kit (Ion Torrent Platform)
• Multiplexing Index Primers (Illumina Platform)
• Multiplexing Unique Dual Index Primers (Illumina Platform)

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