Newsletter Synaptic Systems October 2023 - 코아사이언스

A protein familiy with a bad reputation

 

Amyloid beta precursor like protein 1 and 2 (APLP1 and 2) and amyloid precursor protein (APP) belong to one protein family that all share considerable sequence homology in the amino- and carboxyl termini. APLP1 and APLP2 are processed by alpha-, beta-, and gamma-secretase like APP, but in contrast to APP, they do not contain the notorious Abeta motif. Both APLPs function as synaptic adhesion molecules (SAMs) and are involved in synaptogenesis. While APLP1 is expressed only in neuronal cells, APLP2 shows a more ubiquitous expression profile and recent data suggests that this protein also has a function in the immune response, glucose and insulin homeostasis, and cancer progression.
We have introduced excellent new KO-validated mouse monoclonal antibodies against APLP1 and APLP2.

 

Indirect immunostaining of formaldehyde fixed rat hippocampus neurons with mouse anti-APLP1 antibody (cat. no. 489 011, dilution: 1:500, red) and rabbit anti-MAP2 antibody (cat. no. 188 002, dilution 1:1000, green). Nuclei have been visualized by DAPI staining (blue)​.

Indirect immunostaining of formaldehyde fixed rat hippocampus neurons with mouse anti-APLP2 antibody (cat. no. 490 011, dilution: 1:500, red) and rabbit anti-MAP2 antibody (cat. no. 188 002, dilution 1:1000, green). Nuclei have been visualized by DAPI staining (blue)​.

 

Rat monoclonal antibody against murine FoxP3

 

The transcription factor FoxP3, also known as scurfin, belongs to the family of forkhead transcription factors and controls the differentiation and function of regulatory T-cells (Tregs). Expression of FoxP3 is induced by strong and persistent T cell receptor (TCR) signaling. Increasing evidence suggests that FoxP3 is expressed not only in Tregs, but also in a variety of tumor cells. The role of FoxP3 in tumor cells is not yet fully understood, as there is evidence for both a tumor-promoting and tumor-suppressing function.
This new antibody recognizes murine FoxP3 and, to a lesser extent, FoxP3 from rats, but not human FoxP3. It has been tested in IHC, IHC-P and IHC-fresh frozen applications and achieved outstanding results. 

 

Indirect immunostaining of a formaldehyde fixed mouse ileum section with rat anti-FoxP3 antibody (cat. no. HS-491 017, dilution 1:500, red) and rabbit anti-CD4 antibody (cat. no. HS-360 108, dilution 1:100, green). Nuclei have been visualized by DAPI staining (blue). Antigen retrieval (10mM citrate, pH 6.0, overnight at 60°C) has been applied before staining.​

 

Further new products we want to to bring to your attention:

• rat monoclonal antibody against Brn3a, a transcription factor involed in neuronal development.
• recombinant monoclonal rabbit antibody directed against P2Y12, a purinergic receptor relevant for microglia activation.
• recombinant monoclonal rabbit antibody against GABA-A receptor alpha1, coupled to ATTO 488, suitable for live staining.
• recombinant monoclonal chicken antibody against VGLUT1, a glutamate transporter in the membrane of synaptic vesicles.
• recombinant monoclonal rabbit antibody against alpha-Tubulin.
• recombinant monoclonal chicken antibody against RFP, recognizes also mRFP, mCherry, mOrgange2, tdTomato, mScarlet.

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