[Culture Kit for Human NK Cells from PBMC] BINKIT for NK cells espansion [N501-1, N501-2, N501-4]_Biotherapy Institute of Japan/Funakoshi - 코아사이언스

BINKIT for NK Cells Expansion from PBMCs

NK 세포를 편리하고 쉽게 대량 배양 할 수 있는 키트

 Manual of BINKIT® (PDF)

MSDS: Medium (PDF file), Flask (PDF file)

 

사람 말초혈액단핵세포 (PBMCs; Peripheral Blood Mononuclear Cells) 중, 포함된 NK 세포(Natural Killer Cell)가 

더 성장할 수 있게 최적화 된 배양 키트로서 배지 및 플라스크 배양을 통해 대량으로 높은 활성의 NK 세포를 얻을 수 있습니다.

 

※ 본 제품은 연구용이므로 임상 용도로 사용할 수 없습니다.

※ 본 제품은 주문접수 후, 생산되며 납기는 통상 5~6주 입니다.

 

Features
・Natural killer (NK) cells can be expanded from PBMCs without feeder cells.

- NK 세포의 정제 및 배양보조세포 사용 없이 배양 할 수 있습니다.
・The number of NK cells will increase several hundred to several thousand-fold in 3 weeks from PBMCs of healthy donor. 

NK cells will gain strikingly enhanced cytotoxic activity.

* 암 환자의 혈액 세포의 경우, 혈액 상태에 따라 증식 배율이 저하됩니다.
・This kit is suitable for PBMCs from 20 - 50ml of whole blood.

- 20~50 ml의 전혈에서 분리한 PBMC에서 고활성인 다량의 NK 세포를 얻을 수 있습니다.
・Xeno-free (free from animal-derived materials) - 배양 키트는 동물 유래 단백질을 포함하지 않습니다.

 

Reference 
Xuewen Deng, Hiroshi Terunuma, Mie Nieda, Weihua Xiao, Andrew Nicol, Synergistic cytotoxicity of ex vivo expanded natural killer cells in combination with monoclonal antibody drugs against cancer cells, Int Immunopharmacol 14 (2012) 593-605
PMID: 23063974

Chu Thi Thao, Do Thi Hoai Thu, Bui Viet Anh, Truong Linh Huyen, Nguyen Van Phong, Nguyen Thanh Liem, and Hoang Thi My Nhung: Efficient Expansion of Human Umbilical Cord Blood- Derived NK Cells Ex Vivo without Requiring Feeder Layers, Biomed J Sci & Tech Res, 8: 6739-6744 DOI: 10.26717/BJSTR.2018.08.001711 

Hoang Thi My Nhung, Bui Viet Anh, Truong Linh Huyen, Doan Trung Hiep, Chu Thi Thao, Phung Nam Lam, Nguyen Thanh Liem: Ex vivo expansion of human peripheral blood natural killer cells and cytotoxic T lymphocytes from lung cancer patients, Oncol Lett, 15: 5730-5738, 2018 

鄧学文、芦葉恵介、照沼篤、高根翼、鮫島葉月、贄田美江、照沼裕: 培養NK細胞の投与による体内NK細胞の活性化. *Medical Science Digest* 41, 42-45, 2015. 

Deng X, Terunuma H, Terunuma A, Takane T, Nieda M: Ex vivo-expanded natural killer cells kill cancer cells more effectively than ex vivo-expanded gd T cells or ab T cells. Int Immunopharmacol 22: 486-491, 2014 

Terunuma H, Deng X, Nishino N, Watanabe K: NK cell-based autologous immune enhancement therapy (AIET) for cancer. Stem Cells Regenerative Med 9: 9-13, 2013 

Deng X, Terunuma H, Nieda M, Xiao W, Nicol A: Synergistic cytotoxicity of ex vivo expanded naturel killer cells in combination with monoclonal antibody drugs against cancer cells. Int Immunopharmacol 14: 593-605, 2012

 

Ordering Information 

Product Name	Size	Cat.#	Storage	Price
BINKIT for NK Cells Expansion from PBMCs	1kit	N501-1	4℃, Dark	Contact us
BINKIT for NK Cells Expansion from PBMCs	2kits	N501-2	4℃, Dark	Contact us
BINKIT for NK Cells Expansion from PBMCs	4kits	N501-4	4℃, Dark	Contact us

 

* FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC USE.

coresciences 코아사이언스 Funakoshi Korea distributor 후나코시 한국대리점 Biotherapy Institute of Japan 세포치료연구 자연살상세포 증식 배양 배지 키트 natural killer cell cancer therapy immunotherapy immune cell  Biotherapy Institute of Japan Ltd.