[Tetra-Ubiquitin] K48 Tetra-Ubiquitin [SBB-UP0070]_South Bay Bio - 코아사이언스

K48 Tetra-Ubiquitin

Cat.# SBB-UP0070 / Size: 25ug

 

This K48 linked tetra-ubiquitin was recombinantly expressed in E. coli, enzymatically conjugated, and purified via liquid chromatography.

Description

The array of cellular processes initiated and regulated by ubiquitin has been partially explained by the structural diversity of differently linked ubiquitin chains. In a ubiquitin chain, ubiquitin moieties can be conjugated through one of their lysine residues (K6, K11, K27, K29, K33, K48 and K63) or the N-terminal methionine residue (M1), offering countless possibilities to assemble a specific polymer. Ubiquitin molecules can also be modified by other post-translational modifications, including acetylation and phosphorylation, adding another layer of ubiquitin signal regulation and diversification. This K48 linked tetra-ubiquitin was recombinantly expressed in E. coli, enzymatically conjugated, and purified via liquid chromatography.

Specifications

Molecular Weight:	34kDa
Purity:	>95% by SDS-PAGE
Storage Buffer:	50 mM HEPES pH 7.5
Storage	Store at −80°C after product arrival. Avoid multiple freeze / thaws. It is recommended to make multiple aliquots after the first thaw.

 

Figure & Data

K48-Linked Tetra-Ubiquitin, human recombinant

Figure 1. K48-Linked Tetra-Ubiquitin, SDS-PAGE From left to right, increasing amounts of tetra-ubiquitin loaded onto a 10-20% SDS-PAGE gel, stained with coomassie brillant blue. Purity is > 95%.

 

Citations & References

1) Dikic, I., Wakatsuki, S., & Walters, K. J. (2009). Ubiquitin-binding domains — from structures to functions. Nature Reviews Molecular Cell Biology, 10(10), 659–671. https://doi.org/10.1038/nrm2767

2) Akutsu, M., Dikic, I., & Bremm, A. (2016). Ubiquitin chain diversity at a glance. Journal of Cell Science, 129(5), 875–880. https://doi.org/10.1242/jcs.183954

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코아사이언스 coresciences South bay bio 한국 대리점 Ubiquitin proteasome system Real-time TR-FRET conjugation assays cryptases chemistry UB conjugation UB deconjugation C-terminal derivatives UBL derivatives inhibitors cryptate donors cryptate acceptors cryptate kits Di-ubiquitin tetra-ubiquitin immunoproteasomes 20S proteasomes substrates E1s E2s E3s ubiquitinated substrates DUBS