Aphidicolin [HB3690][CAS no. 38966-21-1]_Hello Bio - 코아사이언스

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Aphidicolin

Cat.# HB3690 / Size: 5mg, 25mg

 

Product overview

Name	Aphidicolin

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Description

DNA replication inhibitor. Useful for cell synchronization

Alternative names

APC, APH, Aphidicoline, (+)-Aphidicolin, NSC234714, BRN4689958, ICI69653

Purity

>98%

Biological Data

Biological description

Overview Aphidicolin is a potent DNA replication inhibitor which is often used to achieve cell synchronization. ​ Mechanism Aphidicolin is a potent and specific inhibitor of B-family DNA polymerases and binds at or near the nucleotide-binding site. It prevents DNA polymerase-α from binding dNTPs without blocking the activity of DNA polymerase β or δ. ​ Aphidicolin inhibits DNA replication and some forms of DNA repair. During cell culture, addition of aphidicolin induces cell cycle pause at the G1/S border. DNA synthesis stops in cells that have entered S-phase, while nondividing cells are unaffected. ​ Uses Aphidicolin acts synergistically with vincristine and doxorubicin. In addition to its anti-mitotic effects, it exhibits antibiotic and antiviral activities.

Solubility & Handling

Storage instructions

+4°C

Solubility overview

Soluble in DMSO (25 mM)

Handling

This compound is light sensitive; exposure to light may affect compound performance. We therefore recommend storing the material in the dark and protecting from light. Do not store the material in solution; make up solutions and use immediately.

Important

This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.

Chemical Data

Chemical name

(3R,4R,4aR,6aS,8R,9R,11aS,11bS)-4,9-bis(hydroxymethyl)-4,11b-dimethyltetradecahydro-8,11a-methanocyclohepta[a]naphthalene-3,9-diol

Molecular Weight

338.5

Chemical structure

Molecular Formula

C20H34O4

CAS Number

38966-21-1

PubChem identifier

457964

SMILES

C[C@]12CC[C@H]([C@@]([C@@H]1CC[C@@H]3[C@@]24CC[C@@]([C@H](C3)C4)(CO)O)(C)CO)O

Source

Isolated from Phoma sp. BS 7210

InChi

InChI=1S/C20H34O4/c1-17(11-21)15-4-3-13-9-14-10-19(13,7-8-20(14,24)12-22)18(15,2)6-5-16(17)23/h13-16,21-24H,3-12H2,1-2H3/t13-,14+,15-,16+,17-,18-,19-,20-/m0/s1

InChiKey

NOFOAYPPHIUXJR-APNQCZIXSA-N

MDL number

MFCD00083214

Appearance

White to off-white solid

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References for Aphidicolin

References are publications that support the biological activity of the product

• Structural basis for inhibition of DNA replication by aphidicolin.
• Baranovskiy et al (2014) Nucleic Acids res. 42(22) : 14013-21 Read more (PubMedID: 25429975)
• Cell synchronization by inhibitors of DNA replication induces replication stress and DNA damage response: analysis by flow cytometry.
• Darzynkiewicz et al (2011) Methods Mol Biol. 761 : 85-96 Read more (PubMedID: 21755443)
• Inhibitor analysis of calf thymus DNA polymerases alpha, delta and epsilon.
• Wright et al (1994) FEBS lett. 341(1) : 128-30 Read more (PubMedID: 8137912)
• Mechanism of DNA polymerase alpha inhibition by aphidicolin.
• Sheaff et al (1991) Biochemistry 30(35) : 8590-7 Read more (PubMedID: 1909569)
• Aphidicolin inhibits the synthesis and joining of short DNA fragments but not the union of 10-kilobase DNA replication intermediates.
• Lonn et al (1983) Proc Natl Acad Sci U S A. 80(13) : 3996-9 Read more (PubMedID: 6408640)

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