Newsletter Synaptic Systems January 2023 - 코아사이언스

 

CD31 – a well-established marker for endothelial cells

CD31, also termed PECAM-1, is a highly glycosylated protein that function as a cell-cell-adhesion. It is expressed on the surface (especially at cell-cell junctions) of endothelial cells, platelets, macrophages and Kupffer cells, granulocytes, lymphocytes (T cells, B cells, and NK cells), megakaryocytes, and osteoclasts. Besides being a important factor in endothelial cell-cell- adhesion, it has also a function in other processes, like angiogenesis, (neuro)inflammation, atherosclerosis and cancer metastasis. 
The new rat monoclonal antibody is excellent in WB, IHC and FFPE (IHC-P) applications. It also had the advantage to recognize mouse and rat CD31, but to be negative for human CD31. That makes this antibody a valuable tool for PDX research. 

Indirect immunostaining of PFA fixed mouse brain section (cortex) with rat anti-CD31 (cat. no. HS-351 117, dilution 1:500, red) and anti-Aquaporin4 (cat. no. 429 006, dilution 1:500, green).. Nuclei have been visualized by DAPI staining (blue).

Claudin-5 – gatekeeper of the blood-brain-barrier (BBB)

Claudin-5 is predominantly expressed in endothelial cells, especially at the tight junctions of brain endothelial cells and therefore it is thought that the paracellular permeability of the blood–brain barrier is largely determined by the expression levels of Claudin-5. Recent studies implicate that a down-regulation of Claudin-5 supports pathological conditions like Alzheimer's disease, neuroinflammation, glioblastoma and schizophrenia.
This new guinea pig polyclonal antibody works perfect in WB, ICC, IHC-fresh frozen and FFPE (IHC-P) applications and is ideally suited for co-staining experiments. 

 

Indirect immunostaining of methanol fixed dog epithelial cells (MDCK cells) with guinea pig anti-Claudin-5 antibody (cat. no. 482 005, dilution 1:500, red). Nuclei have been visualized by DAPI staining (blue).​

Tbr2 – activator of neurogenesis 

Tbr2 also known as Eomes or Eomesdermin is a T-domain transcription factor playing a crucial role during brain development. There are three stages of cellular development for neurons in the cerebral cortex: radial glia, intermediate progenitors, and postmitotic projection neurons.  Radial glia express Pax6, while intermediate progenitor cells express Tbr2, and postmitotic projection neurons express Tbr1. Eomes/Tbr2 has also function in adult neurogenesis and memory formation in the hippocampus as shown in Tbr2 KO-systems. Furthermore, this transcription factor plays a role in the differentiation of CD8+ T-cells during immune response and cardiac development. 
So with the new polyclonal guinea pig anti-Tbr2 antibody we closing our "gap" between Pax6 and Tbr2 with an antibody that has superior results in ICC and IHC applications.

Indirect immunostaining of PFA fixed mouse brain (P0) subventricular zone section with guinea pig anti-Tbr2 antibody (cat. no.. 483 005, dilution 1:500, red) and rabbit anti-Tbr1 antibody (cat. no. 328 003, dilution 1:500, green). Nuclei have been visualized by DAPI staining (blue).

More and more we are adding recombinant antibodies to our portfolio. They have several advantages compared to "conventional" polyclonal antibodies, like e.g.: no-batch-to-batch variations or animal-free production. Here are the newest products in this fast growing group:

 

• Anti-beta3-tubulin, a recombinantly produced monoclonal rabbit antibody
• Anti-VAChT, a recombinantly produced monoclonal rabbit antibody for the acetylcholine transporter protein in the membrane of synaptic vesicles
• Anti-Neurofilament L, a recombinantly produced monoclonal rabbit antibody 

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