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PerfeCTa MultiPlex qPCR SuperMix [95108-200, 95108-01K, 95063-200, 95063-01K]_Quantabio - 코아사이언스

코피디 2022. 10. 31. 17:33

PerfeCTa MultiPlex qPCR SuperMix

Sensitive and robust multiplex qPCR assay performance with user-friendly 1-tube reagents

Features & Benefits

  • Available in two versions: Universal and Low-ROX
  • Maximum assay sensitivity and target precision with stringent, ultrapure, and high-yielding AccuStart II hot start Taq DNA polymerase
  • Broad linear detection range with cDNA or genomic DNA templates
  • Enables highly sensitive 4 target detection assays with coamplified, robust internal positive controls
  • Supports efficient vortex mixing with proprietary anti-foaming technology

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PerfeCTa Multiplex qPCR SuperMix is intended for molecular biology applications. 

This product is not intended for the diagnosis, prevention or treatment of a disease.

Product
Kit Size
Cat.#
PerfeCTa MultiPlex qPCR SuperMix Low ROX
200 x 50 μL rxns (4 x 1.25 mL)
95108-200
1000 x 50 μL rxns (1 x 25 mL)
95108-01K
PerfeCTa MultiPlex qPCR SuperMix
200 x 50 μL rxns (4 x 1.25 mL)
95063-200
1000 x 50 μL rxns (1 x 25 mL)
95063-01K

Description 

PerfeCTa Multiplex qPCR SuperMix is a 2X concentrated, ready-to-use reaction cocktail that contains all the necessary components except: primers, probe(s), and DNA template for highly-multiplexed, real-time quantitative PCR. This reagent formulation pushes the boundary of multiplex qPCR by enabling unbiased amplification of up to 5 targets in a single amplification reaction. Suppression of low abundance targets by high abundance reference targets during co-amplification is a common problem in multiplex PCR in which individual assay sensitivity can be significantly compromised. PerfeCTa Multiplex qPCR SuperMix, Low ROX delivers assay performance with exceptionally broad, linear detection and limit-of-detection (LOD) sensitivity to multiplexed qPCR that is comparable single-plex assay performance without the need for rigorous titration of individual primer assays. A key component of this SuperMix is ultra-pure AccuStart™ hot start Taq DNA polymerase that is completely arrested prior to the initial PCR denaturation step. Upon heat activation at 95°C, the antibodies are rapidly and irreversibly denatured, releasing a fully active high-yielding Taq DNA polymerase mutant. This enables specific and efficient primer extension with the convenience of ambient room-temperature reaction assembly.

​

Details 

Contents

2X concentrated SuperMix Reagent, containing:

  • Reaction buffer with multiplex qPCR-optimized MgCl2, dATP, dCTP, dGTP, and dTTP.
  • Ultra-pure AccuStart hot start Taq DNA Polymerase.
  • Reference dye (if applicable).
  • Proprietary performance enhancing additives and stabilizers.

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Storage & Handling

Store components in a constant temperature freezer at -25°C to -15°C protected from light upon receipt.

For lot specific expiry date, refer to package label, Certificate of Analysis or Product Specification Form.

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Instrument Capability

ROX

  • Applied Biosystems 5700
  • Applied Biosystems 7000
  • Applied Biosystems 7300
  • Applied Biosystems 7700
  • Applied Biosystems 7900
  • Applied Biosystems 7900HT
  • Applied Biosystems 7900 HT Fast
  • Applied Biosystems StepOne™
  • Applied Biosystems StepOnePlus™

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Low ROX

  • Applied Biosystems 7500
  • Applied Biosystems 7500 Fast
  • Stratagene Mx3000P®
  • Stratagene Mx3005P™
  • Stratagene Mx4000™
  • Applied Biosystems ViiA 7
  • Applied Biosystems QuantStudio™
  • Agilent AriaMx
  • Douglas Scientific IntelliQube®

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No ROX

  • Quantabio Q
  • BioRad CFX
  • Roche LightCycler 480
  • QIAGEN Rotor-Gene Q
  • Other

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Bio-Rad iCycler iQ systems

  • BioRad iCycler iQ™
  • BioRad MyiQ™
  • BioRad iQ™5

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Performance Data

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코아사이언스 coresciences Quantabio 퀀타바이오 미국 Funakoshi real-time quantitative PCR next generation sequencing reverse transcription PCR qPCR

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