[Diffusive Thermoprobe] Ultrasensitive Fluorescent Thermometer for cellular temperature monitoring_세포 내 온도 분포 영상용 형광프로브

Ultrasensitive Fluorescent Thermometer 

for cellular temperature monitoring

The "Diffusive Thermoprobe"

Useful Tools to monitor intracellular temparature

 

 

<Features>

• The Diffusive Thermoprobe is fluorescent polymeric thermometer.

   It enables us to monitor intracellular temperature near mitochondria, Golgi body etc.
• Fluorescence lifetime imaging microscopy is required for

   measuring intracellular temparature following microinjection into cells.
• The spatial and temperature resolutions of Diffusive Thermoprobe are at the diffraction

   limited level (200 nm) and 0.18 – 0.58°C.
• Diffusive Thermoprobe is not affected by cellular environmental conditions such as pH,

   ionic strength, protein etc.

 

<Structure & Functional diagram>

 

• At low temperatures, a thermoresponsive poly-N-n-proplylacrylamide (NNPAM) sequence in

  Diffusive Thermoprobe assumes an extended structure with hydration of amide linkages,

  in which a water-sensitive N-{2-[(7-N, N-dimethylaminosulfonyl)-2, 1, 3-benzoxadiazol-4-yl](methyl)amino}ethyl-N- 

  methylacrylamide (DBD-AA) unit can be quenched by neighbouring water molecules.
• At higher temperatures, which weaken hydration, the polyNNPAM sequence shrinks because of

  the hydrophobic interaction among the NNPAM units, resulting in the release of water molecules

  and strong fluorescence from the DBD-AA unit.
• An ionic potassium 3-sulfopropyl acrylate (SPA) unit enriches the hydrophilicity of Diffusive Thermoprobe

  to prevent interpolymeric aggregation within a cell.

 

<Example Data>

Temperature mapping in living COS7 cells
Confocal fluorescence image (left) and fluorescence lifetime image (right) of Diffusive Thermoprobe.

 

Local heat production near the mitochondria in living COS7 cells
•Temperatures increase near the mitochondria after the inhibition of ATP synthesis by the uncoupler FCCP.
•Heat production by FCCP resulted in an average temperature increase of 1.02 ± 0.17°C (avg. ± s.d., n = 7)
 inside COS7 cells 30min after the chemical stimuli.
•Confocal fluorescence images of Diffusive Thermoprobe (green) and MitoTracker Deep Red FM (red; left)
 and fluorescence lifetime images of Diffusive Thermoprobe (middle andf right).

 

<Reference>
Okabe K, et al. Nat Commun. 2012 Feb 28;3:705.

 

<Orfering Information>    

Product Name	Size	Code	Storage		Price/Detail
Diffusive Thermoprobe	100 ug	FDV-0002	RT		Contact us
Diffusive Thermoprobe is a fluorescent polymeric thermometer for living cells. It diffuses throughout the cells and gives the information about intracellular temperature distribution by fluorescence lifetime imaging microscopy. This product is licensed by Tokyo University.

 NOTICE : ALL PRODUCTS on THIS E-MAIL ARE FOR RESEARCH USE onLY. NOT FOR DIAGNOSTIC USE.

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