[Antigen-specific T cell isolation & staining] MHC I-Strep HLA-A*0201; Prame (VLDGLDVLL) [6-7094-001]_IBA Lifesciences GmbH - 수입/공급 전문 코아사이언스

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MHC I-Strep HLA-A*0201; Prame (VLDGLDVLL)

MHC class I molecule fused to a Twin-Strep-tag® and refolded with a peptide

Cat.# 6-7094-001 / Size: 10ug

Protocol for MHC I Streptamer® fluorescent staining

Protocol for MHC I Streptamer® magnetic isolation

MHC I-Strep HLA-A*0201; Prame(100-108) (VLDGLDVLL) is a recombinantly expressed MHC class I molecule that presents a peptide from a human preferentially expressed antigen in melanoma (Prame). Our MHC I Streptamer® approach is based on our Strep-tag® technology and facilitates reversible antigen-specific T cell labeling. A Twin-Strep-tag® fused to the MHC I-Strep permits multimerization of MHC I complexes on a Strep-Tactin® backbone, resulting in the formation of MHC I Streptamers.

 

For multimerizing MHC I-Streps, Strep-Tactin® TACS Agarose columns, Strep-Tactin® Magnetic Microbeads or fluorophore-conjugated Strep-Tactin® can be used. This allows affinity chromatographic or magnetic isolation or cell staining for flow cytometry analysis and sorting (FACS), depending on the experimental requirements. The addition of biotin reverses the multimerization of MHC class I molecules, leading to their dissociation from the target cells. The resulting label-free cells are highly viable and fully functional.

Specifications

Concentration: See data sheet for specific Lot. No.

Form: Dissolved in DPBS

Peptide Sequence: VLDGLDVLL

Possible Application: Fluorescent cell staining & sorting, magnetic or affinity chromatographic cell isolation

Recommended Usage: 0.2 µg per 2 x 10^6 total cells for fluorescent staining or magnetic isolation

Sterility: Sterile

MHC I Allele: HLA-A*0201

 

Shipping information

Storage: Long-term: -80 °C, short-term: 2-8 °C

Stability: 12 months after shipping

Shipping: Dry ice

 

추천 상품 

[가스 투과성 세포 배양 백] NIPRO Cell Culture Bags [87-352, 87-302] & Connecting Tube [87-360]

[마이크로플레이트 웰에서 효과적인 세포 회수/소형 셀 스크래퍼] Mini Cell Scraper: Best size for 24, 48 and 96 well culture plates [MCS-200]

 

미니 셀 스트래이너/미니 셀 스트레이너/Single cell 분리] Mini Cell Strainers for 5 ml sampling tubes and 15 ml conical tubes [HT-AMS-12502, HT-AMS-14002, HT-AMS-17002] & Mini Cell Strainer II for 1.5 mL/2.0 mL tube and 5 ml sampling tubes [HT-AMS-04002, HT-AMS-04008]

코아사이언스 coresciences IBA Lifesciences GmbH 독일 한국 대한민국 cell selection & expansion exosome isolation protein production & assay cell isolation staining antigen specific T cell isolation & staining T cell stimulation & expansion