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[Antigen-specific T cell isolation & staining] MHC I-Strep HLA-A*0201; CMV pp65 (NLVPMVATV) [6-7001-001]_IBA Lifesciences GmbH - 수입/공급 전문 코아사이언스
코피디 2022. 3. 22. 11:37안녕하세요!
IBA Lifesciences GmbH 수입/공급 업체 코아사이언스 입니다.
자세한 상품 정보 및 관련 문의는 전화 02-858-0328 또는 info@coresciences.co.kr로 문의주시기 바랍니다.
감사합니다!
MHC I-Strep HLA-A*0201; CMV pp65 (NLVPMVATV)
MHC class I molecule fused to a Twin-Strep-tag® and refolded with a peptide
MHC I-Strep HLA-A*0201; CMV pp65(495-503) (NLVPMVATV) is a recombinantly expressed MHC class I molecule that presents a peptide from a human cytomegalovirus (CMV) phosphoprotein (pp65). Our MHC I Streptamer® approach is based on our Strep-tag® technology and facilitates reversible antigen-specific T cell labeling. A Twin-Strep-tag® fused to the MHC I-Strep permits multimerization of MHC I complexes on a Strep-Tactin® backbone, resulting in the formation of MHC I Streptamers.
For multimerizing MHC I-Streps, Strep-Tactin® TACS Agarose columns, Strep-Tactin® Magnetic Microbeads or fluorophore-conjugated Strep-Tactin® can be used. This allows affinity chromatographic or magnetic isolation or cell staining for flow cytometry analysis and sorting (FACS), depending on the experimental requirements. The addition of biotin reverses the multimerization of MHC class I molecules, leading to their dissociation from the target cells. The resulting label-free cells are highly viable and fully functional.
Specifications
Concentration: See data sheet for specific Lot. No.Form: Dissolved in DPBS
Peptide Sequence: NLVPMVATV
Possible Application: Fluorescent cell staining & sorting, magnetic or affinity chromatographic cell isolation
Recommended Usage: 0.2 µg per 2 x 10^6 total cells for fluorescent staining or magnetic isolation
Sterility: Sterile
MHC I Allele: HLA-A*0201
Shipping information
Storage: Long-term: -80 °C, short-term: 2-8 °C
Stability: 12 months after shipping
Shipping: Dry ice
추천 상품
[가스 투과성 세포 배양 백] NIPRO Cell Culture Bags [87-352, 87-302] & Connecting Tube [87-360]
코아사이언스 coresciences IBA Lifesciences GmbH 독일 한국 대한민국 cell selection & expansion exosome isolation protein production & assay cell isolation staining antigen specific T cell isolation & staining T cell stimulation & expansion
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