[SiaFind Kits] SIAFIND™ PAN-SPECIFIC LECTENZ® KIT [SK0501, SK0501B]_Lectenz Bio - 코아사이언스

SIAFIND™ PAN-SPECIFIC LECTENZ® KIT

Cat.#          Product                                                         Size

SK0501     SiaFind™ Pan-Specific Lectenz® Kit                     1mg

SK0501B   SiaFind™ Pan-Specific Lectenz®, Biotinylated Kit   1mg

 

Description

Lectenz® are a novel class of lectin-like, enzyme-derived glycan-targeting affinity reagents engineered by computationally-guided directed evolution. The reagents are highly purified recombinant proteins, each designed to bind a specific glycan structure, and have advantages over naturally occurring lectins in rapid detection and enrichment of glycoconjugates.

SiaFindTM Pan-Specific Lectenz® Kits (Cat #SK0501 and SK0501B) contain a sialic acid affinity reagent for the detection, separation, or enrichment of sialoglycans terminating in Siaα2,3Gal, Siaα2,6Gal, and Siaα2,8Sia commonly found in glycoconjugates (glycoproteins, glycolipids, and oligo- or polysaccharides). It has high affinity and specificity towards sialoglycans in a linkage independent manner (pan-specific). Each kit also includes a 5X binding buffer to ensure maximum reagent specificity and ease of use.

Supplementary Information

Each SiaFindTM Lectenz® has a molecular mass of about 77 kD and works as a monomer without bivalent metal ions. They are 6xHis-tagged proteins and an anti-6xHis antibody, or in the case of the biotinylated version, a streptavidin conjugate can be used for detection.

Form and Storage

The SiaFindTM reagents are supplied lyophilized in 1X SiaFindTM Storage Buffer (50 mM EPPS, 100 mM NaCl, pH 7.5) and should be reconstituted in 100 μL molecular water to yield a 10 mg/mL solution. Concentration is determined by spectrophotometry using E1% 12.7. Once reconstituted, store at 4°C for up to 5 days or -20°C for up to 6 months. Aliquoting is recommended to avoid repeated freeze-thaw cycles.

All 5X buffers should be diluted to 1X with ultrapure water. For instance, to make 250 mL, add 50 mL of any 5X SiaFindTM Binding Buffer to 200 mL water and mix by inversion.  All SiaFindTM Buffers may be stored at 4 to 25°C.

Western Blot

Product Information Sheet

SiaFind Pan-Specific Lectenz Information Sheet

SDS

SiaFind Lectenz SDS

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