[Dyes & Labeling/Fluorescent Dyes/Rhodamine Derivatives/Non-Activated] 5/6-Carboxytetramethylrhodamine NHS ester, mixed isomers [AF-0102][CAS no. 246256-50-8]_emp Biotech - 코아사이언스

 

5/6-Carboxytetramethylrhodamine NHS ester, mixed isomers (5/6-TAMRA-SE)

Cat.# AF-0102-C035.0-001 / Size: 35ug

Cat.# AF-0102-C035.0-003 / Size: 3 x 35ug

Cat.# AF-0102-D005.0-001 / Size: 5mg

Cat.# AF-0102-D020.0-001 / Size: 20mg

Cat.# AF-0102-D025.0-001 / Size: 25mg

Cat.# AF-0102-D100.0-001 / Size: 100mg

Amine-reactive rhodamine dye marker for fluorescence labeling, especially for labeling of biomolecules (Abs: 554 nm, Em: 577 nm).

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Properties

CAS-Number:	246256-50-8
Molecular Weight:	527,5
Empirical Formula:	C29H25N3O7
Absorption [nm]:	554 ± 3
Emission [nm]:	577 ± 3
ε [M-1 cm-1]:	>80 000
Buffer System for Fluorescence Readings:	T&S Buffer, pH 9
Solubility:	Acetonitril, DMF, DMSO
Appearance (colour):	violett
Appearance (form):	solid (powder)
Storage:	- 20 °C. Keep dry! Keep away from light, especially in solution!
Trade name:	5/6-Carboxytetramethylrhodamine, succinimidylester, mixed isomer; 5/6-TAMRA succinimidyl ester, mixed isomer
Reactive Moiety:	NHS ester, reacts with 1° amines to form a stable amide linkage

Description

This single isomer non-activated fluorescein derivatization reagent may be used for covalent labeling of proteins and alkylamino modified nucleic acids.

Fluorescein has an excitation maximum that closely matches the 488 nm spectral line of the argon-ion laser, making it the predominant fluorophore for confocal laser scanning microscopy and flow cytometry applications. It has a relatively high absorptivity, excellent fluorescence quantum yield, and good water solubility. Importantly, protein-fluorescein conjugates are not inordinately susceptible to precipitation.

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5-FAM

Absorption: 493 nm (e = 72.000 M-1cm-1) at pH >8

Emission: 517 nm at pH >8

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* 본 상품은 오직 연구용으로만 사용 가능합니다. 인체 및 제품화에 사용하실 수 없습니다.

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