Chitozen - Adhesive coverslips for bacteria imaging [TMI-CHI-7525-min, TMI-CHI-7525]_IDYLLE - 코아사이언스

Chitozen - Adhesive coverslips for bacteria imaging

Chitosan-coated coverslips for live bacteria imaging

Cat.# TMI-CHI-7525-min / Size: 5 coverslips only

Cat.# TMI-CHI-7525 / Size: 5 coverslips with microfluidic channels

 

​Chitozen is a chitosan-coated coverslip immobilizing bacteria for microscopy without inducing bacteriostatic effects. It is compatible with 6-channel sticky slides for flow experiments. It is very helpful if you want to:

• Image bacteria both still and alive under the microscope
• Maintain bacteria in a same focal plane for imaging while preserving their physiology
• Renew culture medium, change growth condition (e.g. antibiotics, chemicals, inhibitors) during the experiment and directly observe, in real-time, the bacteria new comportment under the microscope

 

Kit contents

Chitozen coverslips are compatible with 6-channel sticky slides to work in a closed system in static or dynamic flow conditions. Order you coverslips alone if you need to work in an open system (i.e. for AFM imaging) or to place them on depression slides.

 

• 5-coverslips alone*
• > 5x standard (25 x 75 mm) chitosan-coated coverslips

• 5-coverslips with microfluidic channels (= 30 experiments)
• > 5x standard (25 x 75 mm) chitosan-coated coverslips

> 5x bottomless 6-channel sticky slides

• (Optional) centrifugation pack: the Chitozen coverslips can be centrifuged using a rack compatible with standard microscope slide (25mmx75mm) dimensions. If needed, add our centrifugation pack to your Chitozen kit. It includes:

> µ-Slide Microscopy Rack (ibidi)

> Magnetic Lid for Microscopy Rack

> Clamp & adapter to securely fasten sticky slides to Chitozen coverslips

*If using the Chitozen coverslips alone, separate wells can be created manually by using a sealing glue or Stencell silicon chambers. We can provide these 2 products for you.

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Applications

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• Assay compatibility:
• > Fluorescence
• > Co-cultures (bacterial predators, immune cells)
• > Addition of external factors (e.g. antibiotics, chemicals, inhibitors)
• > Static or dynamic conditions
• Imaging modes:
• > phase-contrast,
• > epifluorescence,
• > confocal,
• > super-resolution microscopy,
• > atomic force microscopy (AFM) - documentation & example pictures available on request
• Experimental outputs:
• > Behavioural changes: growth, elongation, cell division, fitness, colony/biofilm formation, etc
• > Single molecule imaging
• Chitozen is efficient with the following bacteria:
• > E. coli
• > Bacillus subtilis
• > Caulobacter crescentus
• > Corynebacterium glutamicum
• > Helicobacter pylori
• > Mycobacterium smegmatis
• > Myxococcus xanthus
• > Pseudomonas aeruginosa
• > Pseudomonas fluorescens
• > Salmonella
• > Staphylococcus aureus
• > Vibrio cholerae
• This list is updated regularly according to feedback provided by researchers who use Chitozen.

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Technical specifications

Material: glass

Format: 75.0 x 25.0 mm

Thickness: 0.170 mm / 1.5H

Lifetime: up to 12 months at room temperature, shielded from direct sunlight

Sticky slide dimensions (mm):

 

Additional resources:

> Product overview

> Technical Datasheet

> Safety Datasheet

> Certificate of Analysis - 2310

> Certificate of Analysis - 2406

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Publications

Original publication:

Tréguier, J., Bugnicourt, L., Gay, G., Diallo, M., Islam, S. T., Toro, A., David, L., Théodoly, O., Sudre, G., & Mignot, T. (2019). Chitosan Films for Microfluidic Studies of Single Bacteria and Perspectives for Antibiotic Susceptibility Testing. mBio, 10(4), e01375-19. https://doi.org/10.1128/mBio.01375-19

Additional publications:

Islam ST, Jolivet NY, Cuzin C, Belgrave AM, My L, Fleuchot B, Faure LM, Mahanta U, Kezzo AA, Saïdi F, Sharma G, Fiche JB, Bratton BP, Herrou J, Nollmann M, Shaevitz JW, Durand E, Mignot T. Unmasking of the von Willebrand A-domain surface adhesin CglB at bacterial focal adhesions mediates myxobacterial gliding motility. Sci Adv. 2023 Feb 22;9(8):eabq0619. doi: 10.1126/sciadv.abq0619. Epub 2023 Feb 22. PMID: 36812310; PMCID: PMC9946355.

Santin, Y.G., Sogues, A., Bourigault, Y. et al. Lifecycle of a predatory bacterium vampirizing its prey through the cell envelope and S-layer. Nat Commun 15, 3590 (2024). https://doi.org/10.1038/s41467-024-48042-5

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*본 상품은 오직 연구용으로만 사용 가능합니다. 인체 및 제품화에 사용하실 수 없습니다.

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