[마우스 조혈모세포 배양용 무혈청배양배지] HemEx-Type9A; Serum-free medium w/ PVA for mouse Hematopoietic Stem Cells [87-476] - 코아사이언스

The World's first medium for mouse Hematopoietic Stem Cells 

for maintaining undifferentiated state by Polyvinyl Alcohol (PVA)

HemEx-Type9A

Serum-free medium with PVA for mouse Hematopoietic Stem Cells

Cat.# 87-476 / Size: 100mL

HemEx-Type는 알부민 대체제로서 폴리비닐알코올(PVA)을 함유한 무혈청 세포배양액으로서 쥐 조혈모세포(mouse HSCs) 배양에 사용됩니다. 

일반적인 HSCs 배양 배지에는 혈청 알부민이 포함되어 있습니다. 알부민은 HSCs 세포분화에 필요한 반면 미분화 상태의 안정적 유지를 방해합니다. 

널리 사용되는 정제 혹은 재조합 알부민의 경우, 소량의 불순물들을 함유하는데 이것들은 제거하기가 매우 어렵습니다. 그러한 불순물들은 HSCs의 분화를 유도할 뿐 아니라 단백질 산화 반응을 일으켜 세포 노화를 야기합니다. 따라서 알부민은 다른 안정적인 성분으로 대체할 필요가 있습니다.

 

PVA는 알부민을 대체할 수 있을 뿐만 아니라, 배지 내에서 산화 반응이 없는 안정적인 대체제입니다.

이러한 장점은 미분화 상태를 유지하며 HSCs를 증식 배양하는데 도움을 줍니다.

HemEx-TypeA is a serum-free media, which contains Polyvinyl Alcohol (PVA) and can be used for

mouse Hematopoietic Stem Cells (HSCs).

 

In general, culture media for HSCs contains serum albumin. Such albumin is required to induce cell division

of HSCs. on the other hand, albumin inhibits stable undifferentiated state.

 

Widely used purified or recombinant albumin contains small amount of contaminants, but are tough to remove.

It is reported that such contaminants induce differentiation of HSCs. Moreover, protein's oxidation reaction

induces cellular senescence. Therefore, albumin needs to be replaced by other chemicals.

 

Dr. Satoshi Yamazaki at The Institute of Medical Science, The University of Tokyo reported that

PVA can replace albumin (1). Moreover, PVA is a stable chemical with no oxidation reaction

in medium so aging of HSCs are also suppressed. These advantages help to do expansion culture of

HSCs while maintaining undifferentiated state.

 

(1) Adam C., et al. Nature, 571, 117 - 121 (2019).
     Long-term ex vivo hematopoietic stem cell expansion allows nonconditioned transplantation

Features 

・ Optimized for the expansion of mouse HSCs, Hematopoietic Stem and Progenitor Cell (HSPCs)

    obtained from bone marrow.

・ Can do expansion culture of HSCs, for more than 1 month in undifferentiated state.

・ PVA is used instead of albumin.

Protocol 

Please refer the paper of Nature, 571, 117 - 121 (2019).

Note : For culture, addition of cytokine is required.

Data 

Fig. 1 Flow cytometry analysis of HSCs

HSCs were cultured with HemEx (contains 10 ug/ml of SCF and 100 ug/ml of TPO)

for 7 days in 96 well U-bottom culture plate. Lin-/Sca-1+/c-Kit+ is defined as

undifferentiated mouse HSPCs in this data.

* FOR RESEARCH USE onLY. NOT FOR DIAGNOSTIC USE.

코아사이언스 coresciences Funakoshi Inc. Japan 후나코시 한국 대리점 cell science & technology institute CSTI 마우스 쥐 조혈모세포 폴리비닐 알코올 알부민 대체제 대체시약 안정적 미분화 상태 유지 serum-free media SFM 무혈청배양액 무혈청배지 SERUM FREE MEDIA 

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