[박테리아 세포 용해 버퍼] NZY Bacterial Cell Lysis Buffer [MB17801, MB17802]_NZYtech - 코아사이언스

NZY Bacterial Cell Lysis Buffer

Cat.# MB17801 / Size: 250 ml

Cat.# MB17802 / Size: 2 x 250 ml

Manual / MSDS

NZY Bacterial Cell Lysis Buffer is an innovative product developed by NZYTech for the gentle disruption of Escherichia coli cell wall, generating a homogeneous cell-free extract.

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NZY Bacterial Cell Lysis Buffer is an innovative product developed by NZYTech for the gentle disruption of Escherichia coli cell wall, generating a homogeneous cell-free extract. It provides a rapid and cost-effective alternative to harsh chemicals or mechanical procedures, such as French Press or sonication, to release recombinant and native proteins without denaturation. This extraction reagent is a Tris-buffered formulation (pH 7.5) with lysozyme and DNase I provided separately. The addition of these enzymes allows a most efficient extraction.

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In , protein extraction efficiencies are strain-dependent. NZY Bacterial Cell Lysis Buffer can be used to disrupt most common bacterial host strains and it is especially efficient with BL21 strain and its derivatives. The use of pLysS or pLysE hosts enhances protein extraction efficacies.

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Components:

– NZY Bacterial Cell Lysis Buffer

– Lysozyme (at 50 mg/mL)

– DNase I (at 2 mg/mL)

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Application:

Cell lysis

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Citations:

Physiological characterization of a pyrimidine auxotroph exposes link between uracil phosphoribosyltransferase regulation and riboflavin production in Ashbya gossypii. Silva R, Aguiar TQ, Oliveira C, Domingues L N Biotechnol, 2019.

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Recombinant protein purification and immobilization strategies based on peptides with dual affinity to iron oxide and silica. Aguiar T, Domingues L. Biotechnology Journal, 2023.

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Bare silica as an alternative matrix for affinity purification/immobilization of His-tagged proteins. Freitas I A, Domingues L, Aguiar Q T. Separation and Purification Technology, 2022.

 

Shipping Conditions	Room Temperature
Storage Conditions	15 °C to 25 °C
Application	Cell Lysis
Product Category	Cell preparation & Growth

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