Strep-Tactin® Superflow® high capacity column [2-1209-550, 2-1209-001, 2-1209-051]_IBA Lifesciences GmbH - 수입/공급 전문 코아사이언스

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IBA Lifesciences GmbH 수입/공급 업체 코아사이언스 입니다.

 

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Strep-Tactin® Superflow® high capacity column

Cat.#               Size          

2-1209-550     5 x 0.2ml

2-1209-001     1ml

2-1209-051     5ml

 

2-1209-001

 

Gravity flow column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins

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Description

Strep-Tactin® Superflow® high capacity columns are ready-to-use for purifying strep-tagged proteins below 90 kDa via affinity chromatography. These gravity flow columns contain a highly cross-linked agarose coated with streptavidin variant Strep-Tactin®, which captures proteins fused to a Strep-tag®II or a Twin-Strep-tag®. A straightforward purification principle results into a highly pure (>95%) protein preparation. After regeneration, the columns can be used multiple times.

Please note that biotinylated proteins also bind to Strep-Tactin® Superflow® high capacity. If biotinylated proteins are expected, use BioLock. Furthermore, if large (sample and/or buffer) volumes have to be applied, we recommend our WET FRED system.

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For a resin overview with recommendations, browse our resin specifications.

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Specifications

Bead Size:	60 - 160 µm spherical
Biotin Binding Capacity:	> 900 nmol/ml resin
Dynamic Protein Binding Capacity:	7 mg/ml resin
Exclusion Limit:	6 x 10^6
Form:	Pre-packed gravity flow column
Possible Application:	Protein purification of Strep-tag®II and Twin-Strep-tag® fusion proteins
Specificity:	Twin-Strep-tag®; Strep-tag®II; cross reactivity: biotin
Support:	6% agarose, crosslinked

Shipping information

Storage:	2-8 °C
Stability:	6 months after shipping
Shipping:	Room temperature

Protocol

Protocol protein purification with Strep-Tactin® gravity flow columns

Protocol expression of recombinant proteins in E. coli

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Compatible reagents

Compatible reagents for Strep-Tactin®

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Application notes

Comparison of Strep-Tactin® high capacity with Strep-Tactin®XT

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Videos

Color based functionality check - Strep-Tactin® and Strep-Tactin®XT columns compared

Protein Purification with Large Sample Volumes using Wet Fred

 

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