[가역적 세포질 블루 형광 염색시약/Reversible cytoplasm blue fluorescent dye] CytoSeeing [FDV-0017]_Funakoshi - 코아사이언스

Reversible cytoplasm blue fluorescent dye

CytoSeeing

Cat.# FDV-0017 / Size: 1mg

CytoSeeing는 배지에 첨가하는 것만으로 세포질을 신속하게 염색하는 새로운 가역적 형광 염색 시약입니다. 

염색된 세포질을 형광 관찰 한 후, CytoSeeing을 포함하지 않는 배지로 교환하는 것만으로 세포 내에서 쉽게 형광 염색시약을 제거 할 수 있습니다. 따라서, 연이어 다른 형광 프로브로 세포 염색, 관찰이 용이합니다.

CytoSeeing is a newly developed fluorescent dye, which can stain cytoplasm promptly by just adding

to culture medium. After observation, fluorescent dye can be easily removed by replacing medium

which does not contain CytoSeeing.

 

This product is commercialized based on the research result of Hokkaido University Faculty of Science.

Background

It is well known that morpholgy change of nuclear and cytoplasm are related to differentiation, function

and signal response of cells. Conventional cytoplasm staining dyes are used for cell tracking.

Therefore, once dyes are incorporated into cells, the dyes remain in cytoplasm even after medium change.

The dyes are diluted in the course of cell division, and disappear after 3 to 6 population doubling.

If dyes remained in the cells, it is difficult to observe the cell withother probes after live cell imaging.

 

CytoSeeing overcomes this conventional problem. CytoSeeing can be washed out by just replacing medium without

containing CytoSeeing.

Features 

・ Easy protocol

  - Just add CytoSeeing to culture medium.

・ No staining on nucleus

・ Compatible with green and red fluorescent dyes

  - CytoSeeing can be detected by fluorescence filter for DAPI.

・ Little effect to cell function

・ Removable

  - Just replace with fresh medium without containing CytoSeeing. Cells can be used for further assays.

・ Compatible with both adhesive and suspension cells.

 

Comparison Table

Reagent	Staining Cytoplasm	Check nuclear morphology	Time of introduction	Wash-out
CytoSeeing	Yes	Yes	3 - 9 minutes	Yes
Company A	Yes	No	15 - 60 minutes	No
Company B	Yes	No	15 - 60 minutes	No

Product Information

・ Molecular Formula : C17H12N3

・ Molecular Weight : 258.1

・ Purity : >97%

・ No effect to cell function

・ Ex/Em (*1)：345 nm / 456 nm

・ Solubility : DMSO (*2)

 

*1 : Compatible with DAPI filter.

*2 : 10 mM stock solution is recommended.

Protocol Overview

1. Culture cells

2. Add CytoSeeing solution to medium, with the final concentration from 10 μM to 50 μM.

3. Incubate for a few minutes.

4. Observe cells by fluorescent microscopy.

5. If needed, remove CytoSeeing by replacing with fresh medium (without containing CytoSeeing).

Example Data 

Fig. 1 Multiple staining of Endoplasmic Reticulum (ER) or Golgi Body

A549 cells are stained with ER probe (Magenta) or Golgi probe (Yellow) after staining by CytoSeeing (50 μM).

CytoSeeing (Cyan) stains entire cytoplasmand fluorescence intensity is increased under hydrophobic environment

such as in ER and Golgi Body.

Fig. 2 Incorporation and distribution of CytoSeeing in A549 cells by time course

a) CytoSeeing (10 μM) was added and incubated. CytoSeeing was completely incorporated into cell by 9 minutes.

b) CytoSeeing incorporated cells were washed and incubatedin fresh medium (without CytoSeeing).

    Almost CytoSeeing was removed in 30 minutes.

Fig.3 Emission Intensity of CytoSeeing in aqueous or organic solvent

References

Kamada, et al., PLOS onE, 11：e0160625(2016).

NOTICE : ALL PRODUCTS on THIS E-MAIL ARE FOR RESEARCH USE onLY. NOT FOR DIAGNOSTIC USE.

 

 

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