Genomic DNA Extraction Kit (HMW, Magnetic Beads) [50014, 50015, 50016]_BioDynami - 코아사이언스

 

NGS Ancient DNA Library Prep Kit

Genomic DNA Extraction Kit for Cells (HMW, Magnetic Beads) (Cat.# 50014)

Protocol download

MSDS download

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Genomic DNA Extraction Kit for Blood (HMW, Magnetic Beads) (Cat.# 50015)

Protocol download

MSDS download

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Genomic DNA Extraction Kit for Tissues (HMW, Magnetic Beads) (Cat.# 50016)

Protocol download

MSDS download

Cat.#	50014S	50014L	50015S	50015L	50016S	50016SP*	50016L	50016LP*
Preps	50	200	50	200	50	50	200	200

*With Disposable Pestle and Tube Set

The Genomic DNA Extraction Kit (HMW, Magnetic Beads) provides a reliable and fast process for extracting high molecular weight (HMW) genomic DNA from cells, blood, and tissues using Solid Phase Reversible Immobilization (SPRI) magnetic beads. With our proprietary magnetic beads technology, the kit eliminates the tedious centrifuge steps for columns. The kit provides a reliable and simple approach for high-quality genomic DNA isolation with fast magnetic response time and high binding capacity.

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Cat.# 50014 Genomic DNA Extraction Kit for Cells (HMW, Magnetic Beads)

Cat.# 50015 Genomic DNA Extraction Kit for Blood (HMW, Magnetic Beads)

Cat.# 50016 Genomic DNA Extraction Kit for Tissues (HMW, Magnetic Beads)

The extracted HMW genomic DNA size ranges are dependent on the beads resuspension: 50-150 kb by tube tapping and 40-100 kb by tube vortexing. Purified DNA is recovered at high yield and high purity without RNA contamination. The typical purity ratios of A260/A280 are around 1.8-2.0, and A260/A230 are around 2.2-2.5. Purified HMW genomic DNA is suitable for applications such as long-read sequencing, linked-read genome assembly, long range PCR, optical mapping, and other general applications.

Features

• High molecular weight DNA: 50 kb to 150 kb
• High purity
• Simple magnetic beads method
• No centrifuge needed
• No column needed
• No vacuum needed

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A portion of the extracted genomic DNA samples were loaded on a PFGE gel with a DNA ladder indicated. Sample A: liver tissue; Sample B: intestine tissue; Sample C: whole blood; Sample D: cultured 293T cells.

Cultured Cell samples

Cultured cells are collected and are resuspended in a buffer and then lysed with a lysis buffer, then mixed with beads to bind genomic DNA. The samples are mixed with a buffer and after washing steps, genomic DNA is eluted in the Elution Buffer. The isolated genomic DNA with the magnetic beads is free of contamination such as RNA, proteins, salts, and other impurities.

Blood samples

Whole blood is resuspended in the RBC buffer to remove RBC. The remaining leucocytes are lysed with a lysis buffer, then mixed with beads to bind genomic DNA. The samples are mixed with a buffer and after washing steps, genomic DNA is eluted in Elution Buffer. The isolated genomic DNA with the magnetic beads is free of contamination such as RNA, proteins, salts, and other impurities.

Tissue samples

Tissues are homogenized and lysed, then mixed with beads to bind genomic DNA. The samples are mixed with a buffer and after washing steps, genomic DNA is eluted in Elution Buffer. The isolated genomic DNA with the magnetic beads is free of contamination such as RNA, proteins, salts, and other impurities.

Related Products

• Magnetic beads (DNA & RNA Purification)
• Magnetic beads (PCR Purification)
• cfDNA Purification Kit (Magnetic Beads)
• Plasmid MiniPrep High Throughput Kit (Magnetic Beads)
• Plasmid MiniPrep Kit (Magnetic Beads)

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* 본 상품은 오직 연구용으로만 사용 가능합니다. 인체 및 제품화에 사용하실 수 없습니다.

코아사이언스 coresciences BioDynami Korea distributor 한국 대리점 NGS Library Preparation Targeted Sequencing DNA purification magnetic beads Nucleic acid extrasction DNA RNA Quantification DNA Fragmentation DNA ladder 핵산 분리 정제 추출 시퀀싱 차세대 염기서열 분석 키트 시약 마그네틱 비드