티스토리 뷰

실험.연구용 추천상품

[Ion Torrent Platform] NGS DNA Fragmentation & Library Prep Kit [30052, 30053]_BioDynami - 코아사이언스

코피디 2023. 12. 21. 16:25
 

NGS DNA Fragmentation & Library Prep Kit

Protocol download / MSDS download (Cat.# 30052, Ion Torrent Platform)

Protocol download / MSDS download (Cat.# 30053, Ion Torrent Platform)

 

Cat.#
30052S
30052L
30053S
30053L
Reactions
24
48
48
96

The NGS DNA Fragmentation & Library Prep Kit (ion torrent platform) was developed for construction of high quality libraries for next generation sequencing for ion torrent platform. The kit uses intact genomic DNA (both EDTA-free DNA and DNA in TE buffer are compatible) as input DNA without an additional DNA fragmentation step. Our NGS kit has a fast and simple workflow with only three step. In addition, all steps can be performed in one tube. The DNA libraries can be generated within one hour with less than 10 minutes of hands-on time. Library multiplexing up to 12 samples is possible.

 

NGS DNA Fragmentation & Library Prep Kit Workflow

​

The incorporation of DNA fragmentation in the kit makes it possible to directly use intact genomic DNA as input DNA without the need of mechanical DNA shearing or enzymatic DNA fragmentation. The protocol is optimized to generate libraries from 200 bp to 500 bp in size. The library size is inversely correlated with the incubation time of step 1 at 20°C.

​

Kit features:

  • Fast: 1-hour library construction from intact genomic DNA to NGS library
  • Total time: 1 hr
  • Hands-on time: 5 min
  • Intact genomic DNA can be used directly as input; DNA fragmentation is not required.
  • Works with both EDTA-free DNA samples and DNA samples in TE buffer
  • Simple workflow
  • Three steps
  • Only one beads purification step
  • Guaranteed quality: high library conversion efficiency based on our chemistry

​

Library conversion efficiency for NGS DNA Fragmentation & Library Prep Kit (Ion Torrent Platform). 100 ng, 300 ng and 500 ng of DNA were used as input.

* 본 상품은 오직 연구용으로만 사용 가능합니다. 인체 및 제품화에 사용하실 수 없습니다.

코아사이언스 coresciences BioDynami Korea distributor 한국 대리점 NGS Library Preparation Targeted Sequencing DNA purification magnetic beads Nucleic acid extrasction DNA RNA Quantification DNA Fragmentation DNA ladder 핵산 분리 정제 추출 시퀀싱 차세대 염기서열 분석 키트 시약 마그네틱 비드

'실험.연구용 추천상품' 카테고리의 다른 글

LRC/KIR Targeted Sequencing Kits [32023, 32024]_BioDynami - 코아사이언스  (0) 2023.12.21
[Ion Torrent Platform] NGS Library Quantification Standards With PCR Primers [30062]_BioDynami - 코아사이언스  (0) 2023.12.21
[Ion Torrent Platform] NGS DNA Library Prep Kit [30050, 30051]_BioDynami - 코아사이언스  (0) 2023.12.21
[Illumina platform] NGS Library Quantification Standards With PCR Primers [30012S, 30012L]_BioDynami - 코아사이언  (0) 2023.12.21
[Illumina platform] Multiplexing Index Primers [30072S, 30072L]_BioDynami - 코아사이언스  (0) 2023.12.21