[엑소좀 분리,정제 SEC 컬럼] Exosome Purification Column: EVSecond [501021390, 501021392, 501050450]_Funakoshi - 코아사이언스

EVSecond

Exosome purification column by size exclusion chromatography

본 상품은 엑소좀 (exosome) 정제를 위해 특별히 고안된 사이즈 배제 크로마토그래피 

(SEC; size exclusion chromatography / Total length: 75mm) 컬럼입니다. 

엑소좀을 혈청, 혈장, 그리고 세포배양 상청액으로부터 쉽게 분리할 수 있습니다.

울트라 원심분리기와 같은 특별한 장비가 요구되지 않습니다. 

EVSecond is a specially developed size exclusion chromatography (SEC) column, for Exosome purification.
Exosome can be easily purified from serum, plasma and cell culture supernatant.
Special instrument, such as ultracentrifuge is not required.

Features 

• Easy procedure by free fall
  - Special instruments are not required.
• Best for exhaustive miRNA analysis and high sensitivity proteome analysis
  - Free RNA or protien in sample can be efficiently removed.
• Exosome can be applied to further application
  - Purified exosome do not have structural damage.
• Special rack "GL-SPE EXO Fraction Rack" is available as an option
  - This special rack makes preparation and collection easy.

Example Data 

Fig. 1 Purification of exosome from human serum
Each 100 μL of fraction are collected. By using EVSecond, exosome were purified from 100, 200, 300 or 700 μL human serum.
Exosome (Red) and serum proteins (Blue, e.g. albumin or IgG) are separated accurately.
Red : Exosome fraction site was confirmed by CD9 sandwich ELISA.
Blue : Serum proteins fraction site was confirmed by Bradford method.

Data is kindly provided by Dr. Ueda (University of Tokyo) 

Procedure 

(A) Preparation of Column (To be executed in 4 degree condition)
Mix filler well by inverting column until filler is mixed evenly and completely. 
* Mix the column on the shaker overnight gently in cold room is recommended.
* Filler clumps may cause incorrect fraction. Check no clumps are found before use.

(B) Purification (To be executed in 4 degree condition)

1. Remove two caps (top and bottom) and discard storage buffer.
2. Block the filler by 700 μL of FBS (filtrated by 0.22 μm)
3. Wash 6 times by 700 μL of PBS
4. Apply 50 to 700 μL of sample. Flow all sample completely.
5. Apply 100 μL of PBS twice
6. Apply 750 to 1000 μL of PBS (total added volume from 4 to 6 should be 1000 μL). Flow all volume and discard it.
7. Prepare collection tube, and collect 12 fractions by 100 μL of PBS.

Attention 

* Initially, elution position of exosome and serum proteins should be checked by ELISA (CD9 ELISA, for exosome) or Bradford method (for serum proteins).
* Samples which contain objects and may stuck in the column should be pretreated by following protocol.

1. Centrifuge sample by 12000 x g, for 15 minutes in 4℃.
2. Collect sample from middle layer (50 to 700 μL). Do not take precipitation at the bottom or lipids (upper layer).

Special Rack: GL-EXO Fraction Rack 

EVSecond을 사용할 때 분획 수거 작업을 쉽게 할 수 있도록 고안된 특수 랙입니다. 

• Rack can hold up to 8 collection columns (1.5 mL or 2 mL).
• Collection columns can be shifted by manipulating a lever.
• Up to six sets of columns can be set.
• Size : 300(W)×300(D)×150(H) mm

주문 정보

Product Name	Size	Cat.#	Storage
EVSecond	10 pieces	501021390	4℃
	25 pieces	501021392	4℃
GL-SPE EXO Fraction Rack	1 set	501050450	RT

NOTICE : FOR RESEARCH USE onLY. NOT FOR DIAGNOSTIC USE.

coresciences 코아사이언스 Funakoshi co., ltd. 후나코시 한국 공식대리점 일본 후나코시  GL Sciences Inc.