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Phadebas® DLA2 (Detection of Low Amylase Activity) [1331]_Phadebas AB - 코아사이언스

코피디 2023. 10. 19. 16:18
 

Phadebas® DLA2 (Detection of Low Amylase Activity)

Cat.# 1331

Description

Phadebas® DLA2 is a product for detection and semi-quantification of lower levels of α-Amylase specifically. Could be used e.g. in quality control for α-amylase residues in Food Production Industry and Research.

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A DLA2 dish has 7 sample wells, one for negative control and 6 for samples or references. Liquid samples can be analyzed, independent of composition, coloring and opacity.

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DLA2 is Ready to Use, Easy to Use, Easy to Evaluate and requires low work effort and equipment

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Phadebas® Instructions for Use

Instructions Phadebas® DLA2

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Phadebas® Safety Data Sheets

SDS Phadebas® DLA2 (Detection of Low Amylase Activity)

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Q&A Phadebas® DLA2

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Q: Can I reuse the dish?

A: No, not even if there are non used wells.

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Q:Why am I not getting any clear phase?

A: The amylase activity is below detection level.

– The sample does not contain any α-amylase.

– The α-amylase in the sample has been deactivated.

– No sample has been added.

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Q: How should I dilute the concentrated α-amylase solution before use as a reference?

A; Dilute your α-amylase reference freshly with deionised water before application and

in general, they can be diluted 100 000- 1000 000 times.

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Q: Can I run different samples on the same plate?

A: Yes, if your samples contain the same type of α-amylase and could be detected at the same temperature.

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Q: Why do I get a clear zone in the negative control?

A: Your pipette, pipette tips, water etc. is contaminated with α-amylase.

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Q: What should I do if a clear zone interact with other clear zones?

A: Dilute your sample with deionized water and run your sample ones more. Remember dilution factor.

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Q: Can I analyse opaque and colored samples?

A: Yes, Sample must be in the liquid form, if not dilute with deionised water.

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Q: Why do I not get any detection of my diluted reference sample?

A: It is important that your reference samples are freshly prepared prior to use, due to the fact that α- amylase can lose activity over time.

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Q: Can I incubate the dish for more than 16 hours?

A: Yes, but there is a risk of dehydrating and cracking of the gel.

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Q: Why do I get clear zones not connected to the wells?

A; The dish has been contaminated with α-amylase from undefined source.

The samples should be reanalysed.

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Q; Could bacteria, yeast or mold grow on the dish?

A; No, there is a preservative added to the dish to avoid this.

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Q; Is it possible to detect other amylases or enzymes than α-amylase?

A; No, this dish is specific for α-amylase detection.

* 본 상품은 오직 연구용으로만 사용 가능합니다. 인체 및 제품화에 사용하실 수 없습니다.

코아사이언스 coresciences Phadebas AG 한국 대리점 판매 아밀라아제 시험 테스트 분석 소량 알파 아밀라아제 활성 측정 평가 식품 제조 품질 검사 dish 디쉬 

 

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