[당 잔기 구조 분석] PNGase F PRIME-LY™ ULTRA GLYCOSIDASE (Lyophilized Format) [NZSU-LY]_N-Zyme Scientifics/Funakoshi - 코아사이언스

 

PNGase F PRIME-LY™ ULTRA GLYCOSIDASE (Lyophilized Format) 

Cat.# NZSU-LY /  Size: 100ug                

 

PNGase F PRIME-LY™ ULTRA Glycosidase is optimized and specifically formulated for high resolution imaging in small structures or cells. This enzyme specifically removes N-linked oligosaccharides from glycoproteins. This is a high-performance enzyme that has been shown to require as few as 3,000 cells per replicate with 3-20% coefficient of variation to capture label-free N-glycan profiles (as shown in Angel et. al., J Proteome Res. 2019).

Specification 

Target Coreasparagine-linked (N-linked) oligosaccharides (mannose, hybrid, and complex)

Compatible Glycoprotein	Native and denatured
Source of PNGase F PRIME-LY™ ULTRA	Recombinant, E. coli
Total Protocol Length	30 minutes to 24 hours
Typical Yield	1 Unit of enzyme will completely deglycosylate 10 µg of denatured IgG following incubation for 10 minutes at 37°C. 1 Unit = 1 IUB milliunit.
Quantity	Each standard vial of lyophilized format enzyme contains 100 µg of PNGase F PRIME™ [1,000,000 Units/mL (8.14 mg.mL)] lyophilized from 1X PBS. The final concentration of the enzyme is ultimately determined by the amount of dH2O used in the reconstitution process.
Purity	≥95% as determined by SDS PAGE
Contains Glycerol	No
Compatible with Mass Spec	Yes
Affinity tag for purification	Yes. PNGase F PRIME-LY™ ULTRA contains a His-tag.
Reaction Conditions	In vitro and in situ
Storage	-20°C or 4°C (avoid multiple freeze-thaws)
Shelf Life	1 year
Microbe Free	Yes, lot tested

* PLEASE NOTE: 

For general users who are not Glycobiologists, every glycoprotein substrate is different.  

Therefore results can vary depending on the target protein.

 

Example Data 

N-Glycan Profiling of Culture Cells

Typically, N-glycosylation studies done on cultured cells can require up to millions of cells followed by lengthy preparation to release, isolate, and profile N-glycans. To overcome these limitations, a rapid array-based workflow for profiling N-glycan signatures from cells was developed utilizing the capabilities of PNGase F-PRIME-LY™ ULTRA. This method was adapted from imaging mass spectrometry (IMS) used for on-tissue N-glycan profiling. Using this approach, N-glycan profiles from a low-density array of 8 cell chambers could be reported within four hours of completing cell culture.

Image from  Angel, P. et. al. J. Proteome Res. 2019

coresciences 코아사이언스 funakoshi co., ltd. Japan 일본 후나코시 한국대리점 Peptide:N-glycosidase F amidase of the peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase classN-Zyme Scientifics