Anti-MUC1 antibody, Mouse-Mono(014E), Anti-Mucin1 [FDV-0012B]_Funakoshi - 코아사이언스

Anti-MUC1 antibody (clone : 014E)

Specific to scirrhous stomach cancer

Cat.# FDV-0012B / Size: 100ul

 

Anti-MUC1 (Mucin-1) antibody (clone : 014E) is a monoclonal antibody for tumor marker : Mucin-1, that can be used in immunohistochemistry. Especially, this antibody is specific to scirrhous stomach cancer, which was difficult to detect before.

MUC1 (Mucin-1, also called PEM, PEMT or Episialin) a mucin-type glycoprotein is reported that they are associated with cancer progression and metastasis.

MUC-1 is expressed as hetero dimer of extracellular region (MUC1-N) and extracellular region - cell membrane penetrated region - cytoplasmic tail domain (MUC1-C).

MUC1-N has tandem repeat domain (O-glycosylated site) and cleavage sites, and MUC1-C has several splicing variants.

Most commercially available anti-MUC1 antibodies have been produced from MUC1-N or cleavage sites as immunogen, therefore sometimes it cannot detect MUC-1 depending on its cleavage or glycosylation status.

Clone : 014E is produced from common region among splicing variants in the cytoplasmic tail domain (CTD) of MUC1-C as immunogen. This makes it possible to detect various splicing variants, regardless of MUC-1's structure of extracellular domain.

* This antibody has been commercialized under a license from Kagoshima University.

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Features

• Detects various splicing variants
• Isotype : IgG1 kappa
• Immunogen : Common Region in Cytoplasmic Tail Domain (CTD) of Human MUC1 (CRYVPPSSTDRSPYEKVSAG)
• Reactivity : Human
• Application : Immunohistochemistry (IHC-paraffin，1:2,500～1:5,000), Western Blotting (1：2,500～1：5,000)
• Form : Ascites (Contained 50% glycerol. Preservative-free)

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Example Data

Fig.1 Immunohistochemistry Staining

Sample : Human stomach cancer (Adenocarcinoma Grade I)

Retrieval Method ; Heat-induced epotope removal; HIER (ph6.0 / 30 min)

Dilution : 1 : 2,500

Detection Reagent : ImmPRESS Reagent, Anti-Mouse Ig (Vector Laboratories, #MP-7402-15)

Chromogen : ImmPACT DAB Peroxidase Substrate (Vector Laboratories, #SK-4105)

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Fig.2 Western Blotting

Sample : 5 μg cell lysate in each lane

Dilutiuon : 1 : 2,500

Secondary Antibody : Anti-Mouse IgG, Goat-Poly, HRP (Kirkegaard & Perry Laboratories, #074-1516)

Chemiluminescence Substrate : SuperSignal West Pico (Pierce)

Detection : 1 minute exposure

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References:

1. Yonezawa, S., et al., Gastric Cancer, 15, 370-381 (2012).
2. Yokoyama, S., et al., BMC Cancer, 12, 67 (2012).
3. Kitamoto, S., et al., Oncogene, 32, 4614 (2013).
4. Yokoyama, S., et al., PLoS ONE, 9 (4) :e93760 (2014).
5. Japan Patent 5660486, Patent US8951526 B2

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