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Staphylococcus aureus qPCR Kit

Cat.# MD05441 / Size: 100 reactions

Manual / MSDS

Staphylococcus aureus qPCR Kit is designed for the in vitro detection of S. aureus genomes.

Staphylococcus aureus is a Gram-positive spherically shaped bacterium that is a typical member of the microbiota of the body, frequently found in the upper respiratory tract and on the skin. Although S. aureus usually acts as a commensal of the human microbiota, it can also become an opportunistic pathogen, being a common cause of skin infections including abscesses, respiratory infections such as sinusitis, and food poisoning.

Staphylococcus aureus qPCR Kit is designed for the in vitro detection of S. aureus genomes. The kit is built to have the broadest possible detection profile whilst remaining specific to S. aureus. Thus, this kit has been designed for the specific (inclusivity) and exclusive (exclusivity) in vitro detection of this species. The primers and probe sequences have very high (>95%) homology with a broad range of S. aureus genomes based on a comprehensive bioinformatic analysis with all reference data within the NCBI database. This kit can also detects the following Staphylococcus species: S. argenteus and S. roterodami. Real-time PCR detection methods have been used in several studies to identify S. aureus genomes (Galia L. et al 2019).

If required, a complementary kit for the detection of an endogenous gene of the species from which samples are being extracted is available at NZYTech (see Vet, Food & Pharma and Human). The complementary usage of an Endogenous Detection reaction provides a solid confirmation that nucleic acids were properly extracted from the selected biological matrix. If you require further information or have a specific question about the detection profile of this kit, please send an e-mail to info@nzytech.com and our scientific team will answer your question. This kit is designed to be used by trained users in a suitable molecular biology laboratory environment.

Components:

- Lyo NZYSupreme qPCR master mix (2x)

- qPCR master mix reconstitution buffer

- Lyo PPMix (10x)

- NTC

- Positive Control

- DNA Internal Extraction Control (IEC)

 

Shipping Conditions
Room Temperature
Storage Conditions
-85 °C to -15 °C
Format
Lyophilized
Product Category
Predesigned qPCR assays
Compatibility
All standard Real-time thermal cyclers
Target Species
Staphylococcus aureus
Sample Material
DNA
Protocol time
Approx. 60 min (from extracted NA to result)
Number of targets
Duplex
Internal Control
No
Features
Free automatic data analyzer (Fastfinder)
Detection Method
Probe Based

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