[Seal for immunostaining] Barrier Seal for IHC, Type A [R-CSC091-01]_CS CRIE/Funakoshi - 코아사이언스

Barrier Seal for IHC, Type A

Cat.# R-CSC091-01 / Size: 60 pieces

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Specification

• Material: PET
• Outer: 55×25 mm
• Inner: 47×17 mm
• Height: 0.155 mm
• Recommended volume: 200μL
• Storage: RT

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Seal for immunostaining, Type A

Features

• A water-repellent seal surrounds a tissue section on a slide to prevent outflow and mixing of reagents such as antibodies and PBS on the tissue specimen.
• You can reapply or remove the sticker cleanly (with an easy-to-peel tab) without the hassle of painting and drying like a pen type . It is convenient to put the antibody solution without wiping off the water.
• Adhesiveness is maintained even when used at high temperatures (70°C).
• *When applying, if air (air) enters, press the air to remove it.

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Strong Point

• Easy reposition and peel off by removable-adhesive tape and peel tab
• Retains adhesiveness after overnight (55°C) or when used at 70°C
• No need to wipe off moisture before placing the barrier seal
• Hight: 0.155 mm
• Material: PET (removable adhesive)
• ※If there are air bubbles under the seal, please push it out.

Chemical Resistance

CHEMICALS	CONDITIONS	RESISTANCE
0.1% TritonX-100	-	Good
Methanol	-20°C, 20 min	Good
Acetone	-20°C, 20 min	Good
4% PFA (Paraformaldehyde)	RT, 20 min	Good
1% SDS (Sodium dodecyl sulfate)	RT, 20 min	Good
50% DMSO (Dimethyl sulfoxide)	RT, 20 min	Good
50% DMF (Dimethylformamide)	RT, 1 hr	Good
Xylene (or Xylene substitutes)	RT, 5 min×3	Good
100% Ethanol	RT, 5 min×3	Good
70% Ethanol	RT, 5 min×3	Good
Antigen retrieval in water bath	100°C, 1 hr	Good

 

Performance evaluation at a joint research institute

​At a joint research institute, we evaluated the performance of the product when used for immunostaining.

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Evaluation points

1. Check that the seal does not peel off during the immunostaining process

2. Check that the antibody solution does not run off

3. Attachment and detachment of the seal

4. Reuse of the seal

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Experimental conditions

PBST PBSMT Composition: Composition: Skimmed milk containing 0.1% TritonX 2% 100, phosphate buffered saline phosphate buffered saline containing 0.1% TritonX100

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Summary

• The sticker did not come off with the normal immunostaining procedure. There was also no leakage of the antibody solution.
• Antibody solution is usually recommended for antibody volume, but immunostaining is possible even with the minimum antibody volume if antibody can be conserved.
• The seal for immunostaining can be reused by washing with water.
• Since it has a handle, it is easy to remove from the mount.
• Normally, wipe off excess moisture from the slide glass before placing the antibody solution on it, but it is not necessary to wipe off the excess water on slides with immunostaining seals because they repel moisture.

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Water-repellent seal. The sticker format enclosure prevents leakage and outflow of antibody or buffer solution in immunostaining.

 

Ordering Information

more sizes & more types

A type (# R-CSC091-01) Outer: 55 mm×25 mm Inner: 47 mm×17 mm Height: 0.155 mm Recommended volume: 200μL

B type (# R-CSC092-01) Outer: 25 mm×25 mm Inner: 17 mm×17 mm Height: 0.155 mm Recommended volume: 80μL

C type (# R-CSC093-01) Outer: 55 mm×25 mm Inner: 21.5 mm×17 mm Height: 0.155 mm Recommended volume: 100μL

D type (# R-CSC094-01) Outer: φ11 mm Inner: φ5 mm Height: 0.155 mm Recommended volume: 10μL

E type (# R-CSC095-01) Outer: φ16 mm Inner: φ10 mm Height: 0.155 mm Recommended volume: 40μL

F type (# R-CSC096-01) Outer: φ21 mm Inner: φ15 mm Height: 0.155 mm Recommended volume: 60μL

NOTICE : FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC USE.

 

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