5-HTP(5-Hydroxytryptophan) ELISA Kit [ELK8124]_ELK Biotechnology - 코아사이언스

5-HTP(5-Hydroxytryptophan) ELISA Kit

Cat. # ELK8124 / Size: 48T, 96T

 

Product name:	5-HTP(5-Hydroxytryptophan) ELISA Kit
Reactivity:	General
Alternative Names:	Oxitriptan; Cincofarm; Levothym; Levotonine; Oxyfan; Telesol; Tript-OH; Triptum
Assay Type:	Competitive Inhibition
Sensitivity:	1.77 ng/mL
Standard:	400 ng/mL
Range:	6.25-400 ng/mL
Sample Type:	serum, plasma and other biological fluids
Assay Length:	2h
Research Area:	Enzyme & Kinase;
Test principle:	This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 5-Hydroxytryptophan(5-HTP) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 5-Hydroxytryptophan(5-HTP). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 5-Hydroxytryptophan(5-HTP) in the samples is then determined by comparing the OD of the samples to the standard curve.

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