[Diffusive Thermoprobe] Ultrasensitive Fluorescent Thermometer for cellular temperature monitoring_세포 내 온도 분포 영상용 형광프로브
The "Diffusive Thermoprobe"
Useful Tools to monitor intracellular temparature
<Features>
• The Diffusive Thermoprobe is fluorescent polymeric thermometer.
It enables us to monitor intracellular temperature near
mitochondria, Golgi body etc.
• Fluorescence lifetime imaging microscopy is required for
measuring intracellular temparature following microinjection into cells.
• The spatial and
temperature resolutions of Diffusive Thermoprobe are at the
diffraction
limited level (200 nm) and 0.18 – 0.58°C.
• Diffusive
Thermoprobe is not affected by cellular environmental conditions such as
pH,
ionic strength, protein etc.
<Structure & Functional diagram>
• At low temperatures, a thermoresponsive poly-N-n-proplylacrylamide (NNPAM) sequence in
Diffusive Thermoprobe assumes an extended structure with hydration of amide linkages,
in which a water-sensitive N-{2-[(7-N, N-dimethylaminosulfonyl)-2, 1, 3-benzoxadiazol-4-yl](methyl)amino}ethyl-N-
methylacrylamide (DBD-AA) unit can be quenched by neighbouring
water molecules.
• At higher
temperatures, which weaken hydration, the polyNNPAM sequence shrinks because of
the hydrophobic interaction among the NNPAM units, resulting in the release of water molecules
and strong fluorescence from the DBD-AA unit.
• An ionic
potassium 3-sulfopropyl acrylate (SPA) unit enriches the hydrophilicity of
Diffusive Thermoprobe
to prevent interpolymeric aggregation within a cell.
<Example Data>
Temperature mapping in living COS7
cells
Confocal fluorescence image (left) and fluorescence lifetime
image (right) of Diffusive Thermoprobe.
Local heat production near the mitochondria in
living COS7 cells
•Temperatures increase near the mitochondria after
the inhibition of ATP synthesis by the uncoupler FCCP.
•Heat production by
FCCP resulted in an average temperature increase of 1.02 ± 0.17°C (avg. ± s.d.,
n = 7)
inside COS7 cells 30min after the chemical stimuli.
•Confocal
fluorescence images of Diffusive Thermoprobe (green) and MitoTracker Deep Red FM
(red; left)
and fluorescence lifetime images of Diffusive Thermoprobe
(middle andf right).
<Reference>
Okabe K, et al. Nat
Commun. 2012 Feb 28;3:705.
<Orfering Information>
| Product Name | Size | Code | Storage | Price/Detail | |
| Diffusive Thermoprobe | 100 ug | FDV-0002 | RT | Contact us | |
| Diffusive Thermoprobe is a fluorescent polymeric thermometer for living cells. It diffuses throughout the cells and gives the information about intracellular temperature distribution by fluorescence lifetime imaging microscopy. This product is licensed by Tokyo University. | |||||
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