[Dyes & Labeling/Fluorescent Dyes/Rhodamine Derivatives/Non-Activated] 5/6-Carboxytetramethylrhodamine NHS ester, mixed isomers [AF-0102][CAS no. 246256-50-8]_emp Biotech - 코아사이언스
5/6-Carboxytetramethylrhodamine NHS ester, mixed isomers (5/6-TAMRA-SE)
Cat.# AF-0102-C035.0-001 / Size: 35ug
Cat.# AF-0102-C035.0-003 / Size: 3 x 35ug
Cat.# AF-0102-D005.0-001 / Size: 5mg
Cat.# AF-0102-D020.0-001 / Size: 20mg
Cat.# AF-0102-D025.0-001 / Size: 25mg
Cat.# AF-0102-D100.0-001 / Size: 100mg
Amine-reactive rhodamine dye marker for fluorescence labeling, especially for labeling of biomolecules (Abs: 554 nm, Em: 577 nm).
Properties
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CAS-Number:
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246256-50-8
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Molecular Weight:
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527,5
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Empirical Formula:
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C29H25N3O7
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Absorption [nm]:
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554 ± 3
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Emission [nm]:
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577 ± 3
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ε [M-1 cm-1]:
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>80 000
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Buffer System for Fluorescence Readings:
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T&S Buffer, pH 9
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Solubility:
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Acetonitril, DMF, DMSO
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Appearance (colour):
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violett
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Appearance (form):
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solid (powder)
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Storage:
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- 20 °C. Keep dry! Keep away from light, especially in solution!
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Trade name:
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5/6-Carboxytetramethylrhodamine, succinimidylester, mixed isomer; 5/6-TAMRA succinimidyl ester, mixed isomer
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Reactive Moiety:
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NHS ester, reacts with 1° amines to form a stable amide linkage
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Description
This single isomer non-activated fluorescein derivatization reagent may be used for covalent labeling of proteins and alkylamino modified nucleic acids.
Fluorescein has an excitation maximum that closely matches the 488 nm spectral line of the argon-ion laser, making it the predominant fluorophore for confocal laser scanning microscopy and flow cytometry applications. It has a relatively high absorptivity, excellent fluorescence quantum yield, and good water solubility. Importantly, protein-fluorescein conjugates are not inordinately susceptible to precipitation.
5-FAM
Absorption: 493 nm (e = 72.000 M-1cm-1) at pH >8
Emission: 517 nm at pH >8
* 본 상품은 오직 연구용으로만 사용 가능합니다. 인체 및 제품화에 사용하실 수 없습니다.
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