[Dyes & Labeling/Fluorescent Dyes/Rhodamine Derivatives/Amine-Reactive] 6-Carboxytetramethylrhodamine NHS ester, single isomer [AF-0108][CAS no. 136977-42-9]_emp Biotech - 코아사이언스
6-Carboxytetramethylrhodamine NHS ester, single isomer (6-TAMRA-SE)
Cat.# AF-0108-D005.0-001 / Size: 5mg
Cat.# AF-0108-D020.0-001 / Size: 20mg
Cat.# AF-0108-D100.0-001 / Size: 100mg
Amine-reactive rhodamine dye marker for fluorescence labeling, especially for labeling of biomolecules (Abs: 554 nm, Em: 577 nm).
Properties
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CAS-Number:
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136977-42-9
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Molecular Weight:
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527,5
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Empirical Formula:
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C29H25N3O7
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Absorption [nm]:
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548 ± 3
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Emission [nm]:
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570 ± 3
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ε [M-1 cm-1]:
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>78 000
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Buffer System for Fluorescence Readings:
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PBS, pH 7.4
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Solubility:
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Acetonitril, DMF, DMSO
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Appearance (colour):
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violett
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Appearance (form):
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solid (powder)
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Storage:
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- 20 °C. Keep dry! Keep away from light, especially in solution!
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Trade name:
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6-Carboxytetramethylrhodamine, succinimidylester, single isomer; 6-TAMRA-SE; 6-TAMRA succinimidyl ester, singel isomer
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Reactive Moiety:
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NHS ester, reacts with 1° amines to form a stable amide linkage
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Description
This single isomer amine-reactive red fluorescent derivatization reagent may be used for covalent labeling of proteins and alkylamino modified nucleic acids.
The fluorescence quantum yield of TAMRA conjugates is about one-fourth that of fluorescein conjugates. Nevertheless, TAMRA conjugates often appear brighter than the corresponding fluorescein conjugates due to the fact that TAMRA is readily excited by the 546 nm spectral line from mercury-arc lamps used in most fluorescence microscopes and is intrinsically more photostable than fluorescein. TAMRA is also efficiently excited by the 543 nm spectral line of the green He-Ne laser.
6-TAMRA-SE
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