Strep-Tactin® Superflow® high capacity column [2-1209-550, 2-1209-001, 2-1209-051]_IBA Lifesciences GmbH - 수입/공급 전문 코아사이언스
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Strep-Tactin® Superflow® high capacity column
Cat.# Size
2-1209-550 5 x 0.2ml
2-1209-001 1ml
2-1209-051 5ml
Gravity flow column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Description
Strep-Tactin® Superflow® high capacity columns are ready-to-use for purifying strep-tagged proteins below 90 kDa via affinity chromatography. These gravity flow columns contain a highly cross-linked agarose coated with streptavidin variant Strep-Tactin®, which captures proteins fused to a Strep-tag®II or a Twin-Strep-tag®. A straightforward purification principle results into a highly pure (>95%) protein preparation. After regeneration, the columns can be used multiple times.
Please note that biotinylated proteins also bind to Strep-Tactin® Superflow® high capacity. If biotinylated proteins are expected, use BioLock. Furthermore, if large (sample and/or buffer) volumes have to be applied, we recommend our WET FRED system.
For a resin overview with recommendations, browse our resin specifications.
Specifications
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Bead Size:
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60 - 160 µm spherical
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Biotin Binding Capacity:
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> 900 nmol/ml resin
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Dynamic Protein Binding Capacity:
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7 mg/ml resin
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Exclusion Limit:
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6 x 10^6
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Form:
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Pre-packed gravity flow column
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Possible Application:
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Protein purification of Strep-tag®II and Twin-Strep-tag® fusion proteins
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Specificity:
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Twin-Strep-tag®; Strep-tag®II; cross reactivity: biotin
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Support:
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6% agarose, crosslinked
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Shipping information
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Storage:
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2-8 °C
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Stability:
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6 months after shipping
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Shipping:
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Room temperature
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Protocol
Protocol protein purification with Strep-Tactin® gravity flow columns
Protocol expression of recombinant proteins in E. coli
Compatible reagents
Compatible reagents for Strep-Tactin®
Application notes
Comparison of Strep-Tactin® high capacity with Strep-Tactin®XT
Videos
Color based functionality check - Strep-Tactin® and Strep-Tactin®XT columns compared
Protein Purification with Large Sample Volumes using Wet Fred
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