[당 잔기 구조 분석] PNGase F PRIME-LY™ ULTRA GLYCOSIDASE (Lyophilized Format) [NZSU-LY]_N-Zyme Scientifics/Funakoshi - 코아사이언스
PNGase F PRIME-LY™ ULTRA GLYCOSIDASE (Lyophilized Format)
Cat.# NZSU-LY / Size: 100ug
PNGase F PRIME-LY™ ULTRA Glycosidase is optimized and specifically formulated for high resolution imaging in small structures or cells. This enzyme specifically removes N-linked oligosaccharides from glycoproteins. This is a high-performance enzyme that has been shown to require as few as 3,000 cells per replicate with 3-20% coefficient of variation to capture label-free N-glycan profiles (as shown in Angel et. al., J Proteome Res. 2019).
| Compatible Glycoprotein | Native and denatured |
| Source of PNGase F PRIME-LY™ ULTRA | Recombinant, E. coli |
| Total Protocol Length | 30 minutes to 24 hours |
| Typical Yield | 1 Unit of enzyme will completely deglycosylate 10 µg of denatured IgG following incubation for 10 minutes at 37°C. 1 Unit = 1 IUB milliunit. |
| Quantity | Each standard vial of lyophilized format enzyme contains 100 µg of PNGase F PRIME™ [1,000,000 Units/mL (8.14 mg.mL)] lyophilized from 1X PBS. The final concentration of the enzyme is ultimately determined by the amount of dH2O used in the reconstitution process. |
| Purity | ≥95% as determined by SDS PAGE |
| Contains Glycerol | No |
| Compatible with Mass Spec | Yes |
| Affinity tag for purification | Yes. PNGase F PRIME-LY™ ULTRA contains a His-tag. |
| Reaction Conditions | In vitro and in situ |
| Storage | -20°C or 4°C (avoid multiple freeze-thaws) |
| Shelf Life | 1 year |
| Microbe Free | Yes, lot tested |
N-Glycan Profiling of Culture Cells
Typically, N-glycosylation studies done on cultured cells can require up to millions of cells followed by lengthy preparation to release, isolate, and profile N-glycans. To overcome these limitations, a rapid array-based workflow for profiling N-glycan signatures from cells was developed utilizing the capabilities of PNGase F-PRIME-LY™ ULTRA. This method was adapted from imaging mass spectrometry (IMS) used for on-tissue N-glycan profiling. Using this approach, N-glycan profiles from a low-density array of 8 cell chambers could be reported within four hours of completing cell culture.
coresciences 코아사이언스 funakoshi co., ltd. Japan 일본 후나코시 한국대리점 Peptide:N-glycosidase F amidase of the peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase classN-Zyme Scientifics