UltraRIPA kit for Lipid Raft - New "Solution" for extraction of membrane proteins [F015]_Funakoshi Co.,Ltd. - 코아사이언스

New "Solution" for extraction of membrane proteins

UltraRIPA kit for Lipid Raft

Cat.# F015 / Size: 1kit

 

<알림> 본 상품은 현재 수입 중단 되었습니다!

 

UltraRIPA kit는 기존 RIPA buffer보다 높은 가용 효율을 나타내는 새로운 단백질 용해/용출 버퍼입니다. Lipid raft (일명 Detergent resistant membrane: DRM)처럼 기존에 가용화 및 용출이 어려웠던 막결합 단백질들을 비변성 상태로 용출할 수 있어 기능 분석 (Enzymatic assay, immunoprecipitation 등)에 매우 적합합니다.

 

RIPA buffer is one of the most useful buffers for protein extraction.

RIPA buffer maintains most native structures of proteins and can be applied to various applications.

However, RIPA buffer is not sufficient to extract membrane proteins and membrane-associated proteins concentrated in lipid raft.

 

Lipid raft is a highly specialized microdomain on the lipid bilayer which contains specialized lipids, cholesterol and functional proteins.

These lipid rafts are also called “Detergent Resistant Membrane (DRM)”, as lipid raft-enriched proteins are usually insoluble by mild detergent buffers such as 1% Triton X 100 and RIPA buffer.

Consequently, it was difficult to analyze functions of lipid raft-enriched proteins by conventional RIPA buffer.

BioDynamics Laboratory's newly developed product : the UltraRIPA kit, can efficiently and rapidly extract membrane proteins or membrane-associated proteins enriched in lipid rafts with native structure and function.

UltraRIPA kit includes a totally new buffer not containing protein denaturing detergents, but can extract the DRM which was difficult to extract by conventional RIPA buffer. 

UltraRIPA kit helps to analyze various biological assays of the proteins in lipid raft.

 

 

 

Features  

• Extract membrane / membrane associated proteins enriched in lipid rafts, with native structure and fully retained function.

• Easy Procedure

 

 

Overview of Proocedure 

 

 

 

 

Kit Components  

 

• A buffer - 100ml
• B buffer -   10ml

 

Overview of Advantages  

 

 

Example Data  

 

 

* FOR RESEARCH USE onLY. NOT FOR DIAGNOSTIC USE.

 

추천 상품 

 

 

 

BioMasher SP is a larger type of BioMasher II, disposable homogenizer. 

[일회용 호모게나이저,분쇄기,균질기_BioMasher] BioMasher SP_Disposable homogenizer for efficient and improved homogenization [893161, 893162, 893163, 893164]

 

 

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코아사이언스 coresciences innovative cell technologies Funakoshi Inc. Japan 후나코시 한국 대리점 세포 용해 단백질 용출 용해 protein solubilization solubilizer protein extractant extractor RIPA Lysis Extraction Buffer membrane protein 막단백질 용출 버퍼 용해 버퍼 cell lysis reagent buffer solution membrane lipid raft 지질 땟목 마이크로도메인 막결합 지질 단백질 용출 버퍼 막단백질 기능 분석 연구 비변성 non-denaturing non-denaturation conditions 비변성 조건 리파 버퍼 RIPA buffer 리파버퍼 융해