Rat IFNg(Interferon Gamma) ELISA Kit [ELK1133]_ELK Biotechnology - 코아사이언스

Rat IFNg(Interferon Gamma) ELISA Kit

Cat. # ELK1133 / Size: 48T, 96T

Product name:	Rat IFNg(Interferon Gamma) ELISA Kit
Reactivity:	Rat
Alternative Names:	IFN gamma; IFN-G; IFG; IFI; INFr; IFN; Immune Interferon
Assay Type:	Sandwich
Sensitivity:	5.8 pg/mL
Standard:	1000 pg/mL
Detection Range:	15.63-1000 pg/mL
Sample Type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5h
Research Area:	Cytokine;Infection immunity;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat IFNg. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat IFNg. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat IFNg, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat IFNg in the samples is then determined by comparing the OD of the samples to the standard curve.

코아사이언스 coresciences Korea ELK Biotechnology CO.,LTD ELK (Wuhan) Biotechnology CO.,Ltd. 한국 대리점 ELISA antibody 항체 면역학 연구 세포배양 분자생물학 시약